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BAK抗体 [N1N2], N-term

Orthogonal
Anti-BAK antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100063
Anti-BAK antibody [N1N2], N-term used in Western Blot (WB). GTX100063
Anti-BAK antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100063

目录号 GTX100063

文献引用 8
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 BAK 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

23 kDa. ( Note )

预测种属反应

Bovine, Dog, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human BAK. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240507

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BCL2 antagonist/killer 1 , BAK , BAK-LIKE , BCL2L7 , CDN1

细胞定位

Membrane

功能与背景

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-BAK antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100063

GTX100063 IHC-P Image

BAK antibody [N1N2], N-term detects BAK protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
BAK stained by BAK antibody [N1N2], N-term (GTX100063) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BAK antibody [N1N2], N-term used in Western Blot (WB). GTX100063

GTX100063 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BAK antibody [N1N2], N-term (GTX100063) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BAK antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100063

GTX100063 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human liver, bile duct, using BAK1(GTX100063) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
实验应用 : WB
种属反应 : Human
Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
实验应用 : WB
种属反应 : Human
J Exp Med 2021; 218 (10) Enhanced cGAS-STING-dependent interferon signaling associated with mutations in ATAD3A.
实验应用 : WB
种属反应 : Human
Chen YH et al. Mol Med Rep 2021; 23 (1)
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
实验应用 : WB
种属反应 : Human
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产品评论

BAK antibody [N1N2], N-term 目录号 GTX100063

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实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-BAK antibody [N1N2], N-term used in Western Blot (WB). GTX100063
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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