产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HepG2
预测种属反应
Sheep, Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.31 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11177837
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A
细胞定位
Mitochondrion matrix
功能与背景
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2; MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3; MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM]
数据库
研究领域
產品图片
![Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382](/templates/default/js/lightbox/images/loading.gif "Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382")
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GTX111382 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BCKDHA(GTX111382) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111382 ICC/IF Image
BCKDHA antibody [C1C3] detects BCKDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCKDHA protein stained by BCKDHA antibody [C1C3] (GTX111382) diluted at 1:400.
Blue: Hoechst 33342 staining.
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GTX111382 WB Image
Sample (30 μg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX111382 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111382 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [C1C3] (GTX111382) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111382 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [C1C3] (GTX111382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382](/upload/website/prouct_img/small/GTX111382/GTX111382_40506_IHC_w_23060500_563.webp "Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111382](/upload/website/prouct_img/small/GTX111382/GTX111382_40506_20160602_IFA_w_23060500_137.webp "Anti-BCKDHA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382](/upload/website/prouct_img/small/GTX111382/GTX111382_40506_WB_w_23060500_277.webp "Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382](/upload/website/prouct_img/small/GTX111382/GTX111382_45000_20230414_WB_M_R_23103019_116.webp "Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382](/upload/website/prouct_img/small/GTX111382/GTX111382_45000_20230414_WB_23103019_250.webp "Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382](/upload/website/prouct_img/normal/GTX111382/GTX111382_40506_IHC_w_23060500_563.webp "Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111382](/upload/website/prouct_img/normal/GTX111382/GTX111382_40506_20160602_IFA_w_23060500_137.webp "Anti-BCKDHA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382](/upload/website/prouct_img/normal/GTX111382/GTX111382_40506_WB_w_23060500_277.webp "Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382](/upload/website/prouct_img/normal/GTX111382/GTX111382_45000_20230414_WB_M_R_23103019_116.webp "Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382")
![Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382](/upload/website/prouct_img/normal/GTX111382/GTX111382_45000_20230414_WB_23103019_250.webp "Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382")
