产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human BCKDHA.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A
细胞定位
Mitochondrion matrix
功能与背景
The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
数据库
研究领域
產品图片
![Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622](/templates/default/js/lightbox/images/loading.gif "Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622")
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GTX638622 IHC-P Image
BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638622 IHC-Wm Image
BCKDHA antibody [HL2405] detects BCKDHA protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
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GTX638622 WB Image
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (GTX638622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638622 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (GTX638622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638622 ICC/IF Image
BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
目前没有使用 BCKDHA antibody [HL2405] (GTX638622) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 BCKDHA antibody [HL2405] (GTX638622) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622](/upload/website/prouct_img/small/GTX638622/GTX638622_T-45061_20230714_IHC-P_M_23080901_740.webp "Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in IHC (Whole mount) (IHC-Wm). GTX638622](/upload/website/prouct_img/small/GTX638622/GTX638622_45173_20240301_IHC-Wm_Z_24030600_260.webp "Anti-BCKDHA antibody [HL2405] used in IHC (Whole mount) (IHC-Wm). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622](/upload/website/prouct_img/small/GTX638622/GTX638622_45173_20230922_WB_Z_23100118_348.webp "Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622](/upload/website/prouct_img/small/GTX638622/GTX638622_45173_20230922_WB_23100118_620.webp "Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638622](/upload/website/prouct_img/small/GTX638622/GTX638622_T-45061_20230707_ICC_IF_23072519_695.webp "Anti-BCKDHA antibody [HL2405] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622](/upload/website/prouct_img/normal/GTX638622/GTX638622_T-45061_20230714_IHC-P_M_23080901_740.webp "Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in IHC (Whole mount) (IHC-Wm). GTX638622](/upload/website/prouct_img/normal/GTX638622/GTX638622_45173_20240301_IHC-Wm_Z_24030600_260.webp "Anti-BCKDHA antibody [HL2405] used in IHC (Whole mount) (IHC-Wm). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622](/upload/website/prouct_img/normal/GTX638622/GTX638622_45173_20230922_WB_Z_23100118_348.webp "Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622](/upload/website/prouct_img/normal/GTX638622/GTX638622_45173_20230922_WB_23100118_620.webp "Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622")
![Anti-BCKDHA antibody [HL2405] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638622](/upload/website/prouct_img/normal/GTX638622/GTX638622_T-45061_20230707_ICC_IF_23072519_695.webp "Anti-BCKDHA antibody [HL2405] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638622")
