产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human BMAL1.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5
细胞定位
Nucleus,Cytoplasm
功能与背景
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
数据库
研究领域
產品图片
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/templates/default/js/lightbox/images/loading.gif "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
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GTX638774 WB Image
Non-transfected (–) and transfected (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638774 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638774 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638774 WB Image
Untreated and treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638774 IHC-P Image
BMAL1 antibody [HL2456] detects BMAL1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat intestine.BMAL1 stained by BMAL1 antibody [HL2456] (GTX638774) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638774 ICC/IF Image
BMAL1 antibody [HL2456] detects BMAL1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT-116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMAL1 stained by BMAL1 antibody [HL2456] (GTX638774) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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文献引用
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![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/small/GTX638774/GTX638774_T-45089_20230707_WB_shRNA_watermark_23071300_128.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/small/GTX638774/GTX638774_45159_20230908_WB_TPM_watermark_23091319_273.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/small/GTX638774/GTX638774_45159_20231006_WB_M_brain_23102401_449.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/small/GTX638774/GTX638774_T-45089_20240223_WB_treatment_Serumshock_24022619_808.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in IHC (Paraffin sections) (IHC-P). GTX638774](/upload/website/prouct_img/small/GTX638774/GTX638774_T-45089_20230721_IHC-P_R_23073119_156.webp "Anti-BMAL1 antibody [HL2456] used in IHC (Paraffin sections) (IHC-P). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638774](/upload/website/prouct_img/small/GTX638774/GTX638774_T-45089_20230804_ICC_IF_23080901_669.webp "Anti-BMAL1 antibody [HL2456] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/normal/GTX638774/GTX638774_T-45089_20230707_WB_shRNA_watermark_23071300_128.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/normal/GTX638774/GTX638774_45159_20230908_WB_TPM_watermark_23091319_273.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/normal/GTX638774/GTX638774_45159_20231006_WB_M_brain_23102401_449.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774](/upload/website/prouct_img/normal/GTX638774/GTX638774_T-45089_20240223_WB_treatment_Serumshock_24022619_808.webp "Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in IHC (Paraffin sections) (IHC-P). GTX638774](/upload/website/prouct_img/normal/GTX638774/GTX638774_T-45089_20230721_IHC-P_R_23073119_156.webp "Anti-BMAL1 antibody [HL2456] used in IHC (Paraffin sections) (IHC-P). GTX638774")
![Anti-BMAL1 antibody [HL2456] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638774](/upload/website/prouct_img/normal/GTX638774/GTX638774_T-45089_20230804_ICC_IF_23080901_669.webp "Anti-BMAL1 antibody [HL2456] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638774")
