产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse brain , ARNTL transfected 293T cell
预测种属反应
Rat, Sheep(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human BMAL1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1949686
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5
细胞定位
Nucleus
功能与背景
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX105060 WB Image
Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 16109848, 28803917
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GTX105060 WB Image
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX105060 diluted at 1:1000
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GTX105060 WB Image
Western Blot analysis of BMAL1 expression in transfected 293T cell line by BMAL1 polyclonal antibody.
A: Non-transfected lysate.
B: BMAL1 transfected lysate.
7.5% SDS PAGE
GTX105060 diluted at 1:500
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GTX105060 ICC/IF Image
BMAL1 antibody [N1N3] detects BMAL1 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BMAL1 protein stained by BMAL1 antibody [N1N3] (GTX105060) diluted at 1:500.
Blue: Hoechst 33342 staining.
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文献引用
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产品评论
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