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BRCA1抗体 [6B4] - ChIP grade

KO/KD
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunoprecipitation (IP). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in ChIP assay (ChIP assay). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunohistochemistry (IHC). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

目录号 GTX70115

文献引用 3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 6B4

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

种属反应

 Human
产品包装
100 μl (¥4300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

208 kDa. ( Note )

阳性对照

293T , MCF7 , U2OS , HeLa

产品说明

This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

产品属性

形式

Liquid

存储溶液

1XPBS pH7, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_368616

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

细胞定位

Nucleus , Cytoplasm

功能与背景

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

数据库

研究领域

產品图片

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunoprecipitation (IP). GTX70115

GTX70115 IP Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

KO/KD

Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BRCA1 antibody [6B4] - ChIP grade used in ChIP assay (ChIP assay). GTX70115

GTX70115 ChIP assay Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 microgram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunohistochemistry (IHC). GTX70115

GTX70115 IHC Image

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

BRCA1 antibody detects BRCA1 protein by western blot analysis. Whole cell extracts (30 and 50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GTX70115) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

KO/KD

BRCA1 antibody [6B4] (GTX70115) was used at 1:1000 dilution for western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody [13B3] (GTX70228) was used as a loading control.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70115

GTX70115 ICC/IF Image

BRCA1 antibody 6B4 (GTX70115) was used for immunofluorescent staining of BRCA1 nuclear foci induced by ionizing radiation.
IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining.
DAPI was used to counterstain nucleus.
6B4 was used at 1:400 dilution.
Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4).

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

文献引用

Application Reference

Lajud SA et al. Clin Cancer Res 2014; Dual Disruption of DNA Repair and Telomere Maintenance for the Treatment of Head and Neck Cancer.
实验应用 : WB
种属反应 : Human
Bernard-Gallon DJ et al. Anticancer Res 2004; 24 (1) : 321-4 Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
实验应用 : IHC-P
种属反应 : Human
Li S et al. Nature 2000; 406 (6792) : 210-5 Functional link of BRCA1 and ataxia telangiectasia gene product in DNA damage response.
实验应用 : WB, IP
种属反应 : Human
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