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BRCA1抗体 [8F7]

KO/KD
Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113
Anti-BRCA1 antibody [8F7] used in IHC (Paraffin sections) (IHC-P). GTX70113
Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113
Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113
Anti-BRCA1 antibody [8F7] used in IHC (Paraffin sections) (IHC-P). GTX70113

目录号 GTX70113

文献引用 15

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 8F7

同种型

 IgG1

实验应用

 WB, IHC-P, IP, IHC

种属反应

 Human, Mouse
产品包装
100 μl (¥4300)
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产品应用

应用说明

For IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Optimal dilutions/concentrations should be determined by the researcher.

预期分子量

208 kDa. ( Note )

阳性对照

293T

产品说明

This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Protein fragment expressed in E. coli corresponding to amino acids 341-748.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_368614

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

细胞定位

Nucleus , Cytoplasm

功能与背景

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

数据库

研究领域

產品图片

Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113

GTX70113 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [8F7] used in IHC (Paraffin sections) (IHC-P). GTX70113

GTX70113 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Breast ca, using BRCA1(GTX70113) antibody at 1:200 dilution.

Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113

GTX70113 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113

GTX70113 WB Image

KO/KD

The data was published in the journal PLoS One in 2013. PMID: 23805307

Anti-BRCA1 antibody [8F7] used in IHC (Paraffin sections) (IHC-P). GTX70113

GTX70113 IHC-P Image

The data was published in the journal Breast Cancer Res in 2001. PMID: 11250747

文献引用

Application Reference

Lanna A et al. Nat Cell Biol 2022; 24 (10) : 1461-1474 An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.
实验应用 : WB
种属反应 : Human
Dacheux E et al. PLoS One 2013; 8 (6) : e67313 BRCA1-Dependent Translational Regulation in Breast Cancer Cells.
实验应用 : WB
种属反应 : Human
Bosviel R et al. Br J Nutr 2012; 108 (7) : 1187-93 Epigenetic modulation of BRCA1 and BRCA2 gene expression by equol in breast cancer cell lines.
实验应用 : IHC-P
种属反应 : Human
Turner NC et al. EMBO J 2008; 27 (9) : 1368-77 A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor.
实验应用 : WB
种属反应 : Human
Lee MN et al. Clin Cancer Res 2007; 13 (3) : 832-8 Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.
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Application Reference
Lanna A et al. Nat Cell Biol 2022; 24 (10):1461-1474 An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.
实验应用 : WB
种属反应 : Human
Dacheux E et al. PLoS One 2013; 8 (6):e67313 BRCA1-Dependent Translational Regulation in Breast Cancer Cells.
实验应用 : WB
种属反应 : Human
Bosviel R et al. Br J Nutr 2012; 108 (7):1187-93 Epigenetic modulation of BRCA1 and BRCA2 gene expression by equol in breast cancer cell lines.
实验应用 : IHC-P
种属反应 : Human
Turner NC et al. EMBO J 2008; 27 (9):1368-77 A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor.
实验应用 : WB
种属反应 : Human
Lee MN et al. Clin Cancer Res 2007; 13 (3):832-8 Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.
Al-Mulla F et al. J Histochem Cytochem 2005; 53 (5):621-9 BRCA1 gene expression in breast cancer: a correlative study between real-time RT-PCR and immunohistochemistry.
Bernard-Gallon DJ et al. Anticancer Res 2004; 24 (1):321-4 Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
实验应用 : IHC
种属反应 : Human
Okada S et al. J Biol Chem 2003; 278 (3):2015-20 Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization.
Ongusaha PP et al. Oncogene 2003; 22 (24):3749-58 BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.
实验应用 : WB
种属反应 : Mouse
Reed W et al. Int J Surg Pathol 2003; 11 (2):65-74 The prognostic impact of hormone receptors and c-erbB-2 in pregnancy-associated breast cancer and their correlation with BRCA1 and cell cycle modulators.
Chung Y et al. Pakistan Journal of Nutrition. 2002; 1:1 Cloning and expression of bovine BRCA1.
实验应用 : WB
Bernard-Gallon DJ et al. Breast Cancer Res 2001; 3 (1):22402 BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
实验应用 : IHC
种属反应 : Human
Bernard-Gallon DJ et al. Int J Cancer 1999; 82 (5):771-3 Expression of human BRCA1 and BRCA2 proteins in lung from a fetus at 19 weeks' gestation.
实验应用 : IHC
种属反应 : Human
Yoshikawa K et al. Clin Cancer Res 1999; 5 (6):1249-61 Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases.
实验应用 : IHC
种属反应 : Human
Bernard-Gallon DJ et al. Int J Cancer 1999; 81 (5):839-43 BRCA1 and BRCA2 proteins are expressed in milk fat globules.
实验应用 : IHC
种属反应 : Human
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产品包装 报价 (¥)
¥ 4300
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