产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 150mM NaCl, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthesized peptide derived from human BRCA2(Accession P51587), corresponding to amino acid residues E2081-P2107.
纯化方式
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
BRCA2 DNA repair associated , BRCC2 , BROVCA2 , FACD , FAD , FAD1 , FANCD , FANCD1 , GLM3 , PNCA2 , XRCC11
细胞定位
Nucleus
功能与背景
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]
数据库
研究领域
產品图片
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GTX03783 WB Image
WB analysis of various lysates using GTX03783 BRCA2 (phospho Ser2095) antibody.
Lane 1: Rat liver, blocked with antigen-specific peptides.
Lane 2 : Rat liver.
Lane 3 : Mouse brain
Lane 4 : Hela cells (heat-shock treatment).
Lane 5 : HepG2 (heat-shock treatment)
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GTX03783 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX03783 BRCA2 (phospho Ser2095) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX03783 IHC-P Image
IHC-P analysis of human spleen tissue using GTX03783 BRCA2 (phospho Ser2095) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX03783 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX03783 BRCA2 (phospho Ser2095) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX03783 ICC/IF Image
ICC/IF analysis of PFA-fixed HepG2 cells (LPS-treated, 4 hr) using GTX03783 BRCA2 (phospho Ser2095) antibody.
Permeabilize : 0.1% Triton X-100
Dilution : 1:200
Red : Primary antibody
Green : beta-tubulin
Blue : DAPI
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文献引用
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产品评论
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