产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide of human BRD4.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
bromodomain containing 4 , CAP , HUNK1 , HUNKI , MCAP
细胞定位
Nucleus
功能与背景
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/templates/default/js/lightbox/images/loading.gif "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
|
GTX639557 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX639557 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX639557 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX639557 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX639557 ICC/IF Image
BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX639557 IHC-P Image
BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
文献引用
目前没有使用 BRD4 antibody [HL2729] (GTX639557) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 BRD4 antibody [HL2729] (GTX639557) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/small/GTX639557/GTX639557_T-45264_20240126_WB_Fraction_24013018_879.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/small/GTX639557/GTX639557_T-45264_20240126_WB_M_24013018_987.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/small/GTX639557/GTX639557_T-45264_20240126_WB_R_24013018_214.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/small/GTX639557/GTX639557_45369_20240412_WB_24041523_228.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639557](/upload/website/prouct_img/small/GTX639557/GTX639557_T-45264_20240202_ICC_IF_24021917_497.webp "Anti-BRD4 antibody [HL2729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639557")
![Anti-BRD4 antibody [HL2729] used in IHC (Paraffin sections) (IHC-P). GTX639557](/upload/website/prouct_img/small/GTX639557/GTX639557_T-45264_20240301_IHC-P_24030600_472.webp "Anti-BRD4 antibody [HL2729] used in IHC (Paraffin sections) (IHC-P). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240126_WB_Fraction_24013018_879.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240126_WB_M_24013018_987.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240126_WB_R_24013018_214.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557](/upload/website/prouct_img/normal/GTX639557/GTX639557_45369_20240412_WB_24041523_228.webp "Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557")
![Anti-BRD4 antibody [HL2729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639557](/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240202_ICC_IF_24021917_497.webp "Anti-BRD4 antibody [HL2729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639557")
![Anti-BRD4 antibody [HL2729] used in IHC (Paraffin sections) (IHC-P). GTX639557](/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240301_IHC-P_24030600_472.webp "Anti-BRD4 antibody [HL2729] used in IHC (Paraffin sections) (IHC-P). GTX639557")
