产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
K562 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T
预测种属反应
Bovine, Chicken(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2.44 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human BRG1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888340
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b
细胞定位
Nucleus
功能与背景
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/templates/default/js/lightbox/images/loading.gif "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
|
GTX633391 WB Image
Wild-type (WT) and BRG1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX633391 IHC-P Image
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX633391 IHC-P Image
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX633391 IHC-Fr Image
BRG1 antibody [GT2712] detects BRG1 protein by immunohistochemical analysis.Sample: Frozen-sectioned rat E13.5 brain.Green: BRG1 stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.Red: SOX2, stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX633391 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
|
|
|
GTX633391 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
|
|
|
GTX633391 ICC/IF Image
BRG1 antibody [GT2712] detects BRG1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRG1 protein stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
|
|
|
GTX633391 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
|
文献引用
目前没有使用 BRG1 antibody [GT2712] (GTX633391) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 BRG1 antibody [GT2712] (GTX633391) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20180216_WB_KO_watermark_w_23061202_386.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160615_IHC-P_2_w_23061202_163.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160615_IHC-P_w_23061202_117.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20171106_IHC-Fr_R_w_23061202_767.webp "Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20161222_WB_M_w_23061202_107.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160505_WB_w_23061202_653.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160706_IFA_w_23061202_763.webp "Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20161222_WB_R_w_23061202_650.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20180216_WB_KO_watermark_w_23061202_386.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160615_IHC-P_2_w_23061202_163.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160615_IHC-P_w_23061202_117.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20171106_IHC-Fr_R_w_23061202_767.webp "Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20161222_WB_M_w_23061202_107.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160505_WB_w_23061202_653.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160706_IFA_w_23061202_763.webp "Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20161222_WB_R_w_23061202_650.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
