产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Bovine, Chicken, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein of human BTK
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA
细胞定位
Cytoplasm,Nucleus
功能与背景
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]
数据库
研究领域
產品图片
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/templates/default/js/lightbox/images/loading.gif "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
|
GTX637455 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637455 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX637455 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637455 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637455 ICC/IF Image
BTK antibody [HL1803] detects BTK protein by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BTK stained by BTK antibody [HL1803] (GTX637455) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
文献引用
目前没有使用 BTK antibody [HL1803] (GTX637455) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 BTK antibody [HL1803] (GTX637455) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20230616_WB_shRNA_watermark_23062019_226.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20230421_WB_TPM_watermark_23042500_514.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_T-44809_20221021_WB_M_22102723_676.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20221111_WB_22111518_162.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20221230_ICC_IF_23010219_591.webp "Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20230616_WB_shRNA_watermark_23062019_226.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20230421_WB_TPM_watermark_23042500_514.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_T-44809_20221021_WB_M_22102723_676.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20221111_WB_22111518_162.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20221230_ICC_IF_23010219_591.webp "Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455")
