产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000-1:10000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Raji (1 mM pervanadate treatment for 30 min)
预测种属反应
Bovine, Chicken, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr223 of human BTK. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886249
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA
细胞定位
Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Membrane , Nucleus
功能与背景
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX130377 IHC-P Image
BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130377 WB Image
Untreated (–) and treated (+) Raji whole cell extracts (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:6000.
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GTX130377 IHC-P Image
BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130377 IHC-P Image
BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.Sample: Paraffin-embedded rat lymph node.BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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