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Bassoon抗体 [HL1732]

Anti-Bassoon antibody [HL1732] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637380
Anti-Bassoon antibody [HL1732] used in IHC (Paraffin sections) (IHC-P). GTX637380

目录号 GTX637380

宿主

Rabbit

克隆

Monoclonal

克隆编号

HL1732

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

419 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Synthetic peptide of mouse Bassoon

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

bassoon

细胞定位

Cytoplasm,Synapse, synaptosome,Cytoplasm, cytoskeleton

功能与背景

A structural constituent of presynaptic active zone. Involved in presynapse to nucleus signaling pathway; protein localization to synapse; and regulation of synaptic vesicle cycle. Located in several cellular components, including glutamatergic synapse; postsynaptic density; and presynaptic active zone cytoplasmic component. Is active in GABA-ergic synapse and Schaffer collateral - CA1 synapse. Is expressed in nervous system and retina. Used to study epilepsy. Orthologous to human BSN (bassoon presynaptic cytomatrix protein). [provided by Alliance of Genome Resources, Apr 2022]

数据库

研究领域

產品图片

Anti-Bassoon antibody [HL1732] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637380

GTX637380 ICC/IF Image

Bassoon antibody [HL1732] detects Bassoon protein at synapse by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bassoon stained by Bassoon antibody [HL1732] (GTX637380) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Bassoon antibody [HL1732] used in IHC (Paraffin sections) (IHC-P). GTX637380

GTX637380 IHC-P Image

Bassoon antibody [HL1732] detects Bassoon protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Bassoon stained by Bassoon antibody [HL1732] (GTX637380) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min

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产品评论

Bassoon antibody [HL1732] 目录号 GTX637380

Rating ( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-May-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : boiled/unboiled SH-SY5Y and SH-SY5Y membrane extract
Amount used : 30μg
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 :
WCL: whole cell lysate
ME: membrane extract
产品包装 报价 (¥)
¥ 4000
¥ 1700
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