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Bax抗体 [HL236]

Recombinant antibody KO/KD Orthogonal
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715
Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715

目录号 GTX635715

文献引用 3

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL236

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Bax 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

21 kDa. ( Note )

预测种属反应

Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888571

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BCL2 associated X, apoptosis regulator , BCL2L4

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

KO/KD

Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

KO/KD

Wild-type (WT) and Bax knockout (KO) Expi293 cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715

GTX635715 IHC-P Image

Bax antibody [HL236] detects Bax protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Raw264.7 whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715

GTX635715 IHC-P Image

Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715

GTX635715 IHC-P Image

Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Li Y et al. Transl Lung Cancer Res 2021; 10 (4) : 1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
实验应用 : WB
种属反应 : Human
Qi Y et al. Cancer Cell Int 2021; 21 (1) : 74
实验应用 : WB
种属反应 : Human
Huang YY et al. BMC Vet Res 2020; 16 (1) : 193 Inhibiting autophagy potentiates the antitumor efficacy of Euphorbia royleana for canine mammary gland tumors.
实验应用 : WB
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产品包装 报价 (¥)
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