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Bid抗体 [N1C3-2]

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568
Anti-Bid antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110568
Anti-Bid antibody [N1C3-2] used in Immunoprecipitation (IP). GTX110568
Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568
Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

目录号 GTX110568

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IP

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

22 kDa. ( Note )

阳性对照

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Rat2 , Raw264.7 , C2C12

产品说明

IP/MS validation was supported by references (PMID:30377403)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Bid Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949744

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BH3 interacting domain death agonist , FP497

细胞定位

Cytoplasm , Mitochondrion membrane , BH3-interacting domain death agonist p15: Mitochondrion membrane , BH3-interacting domain death agonist p13: Mitochondrion membrane , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Isoform 2: Mitochondrion membrane

功能与背景

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

Bid antibody [N1C3-2] detects Bid protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg H1299 whole cell lysate/extract
D. 30 μg HeLa whole cell lysate/extract
E. 30 μg HepG2 whole cell lysate/extract
F. 30 μg Molt-4 whole cell lysate/extract
G. 30 μg Raji whole cell lysate/extract
7.5 % SDS-PAGE
Bid antibody [N1C3-2] (GTX110568) dilution: 1:1000

Anti-Bid antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110568

GTX110568 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Bid(GTX110568) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bid antibody [N1C3-2] used in Immunoprecipitation (IP). GTX110568

GTX110568 IP Image

Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3-2] (GTX110568) or Bid antibody [N1C3] (GTX101323).
Western blot analysis was performed using Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568

GTX110568 ICC/IF Image

Bid antibody [N1C3-2] detects Bid protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bid protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568

GTX110568 ICC/IF Image

Bid antibody [N1C3-2] detects BID protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: BID protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

The data was published in the 2016 in Oncotarget. PMID: 27564258

文献引用

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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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