产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7 , K562 , THP-1 , 293T , A549 , H1299 , HCT116
预测种属反应
Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036353
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1
细胞定位
Nucleus , Cytoplasm
功能与背景
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
数据库
研究领域
產品图片
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GTX114008 ICC/IF Image
Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm.
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GTX114008 ICC/IF Image
Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX114008 IHC-P Image
Bmi1 antibody detects Bmi1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX114008 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX114008 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 WB Image
Bmi1 antibody detects Bmi1 protein by western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg THP-1 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 WB Image
Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
B. 30 μg HL-60 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 IHC-P Image
Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114008 ICC/IF Image
BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Green: BMI1 protein stained by BMI1 antibody (GTX114008) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX114008 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 IHC-P Image
Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114008 WB Image
Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114008 IP Image
Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 μg of Bmi1 antibody (GTX114008) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX114008) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX114008 WB Image
The data was published in the journal J Biomed Sci in 2016.PMID: 27863490
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GTX114008 WB Image
The data was published in the journal J Biomed Sci in 2016.PMID: 27863490
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GTX114008 WB Image
The data was published in the journal BMC Cancer in 2017.PMID: 28629331
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GTX114008 WB Image
The data was published in the journal Cell Death Dis in 2015. PMID: 26136074
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文献引用
产品评论
Bmi1 antibody
目录号 GTX114008
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Rating
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( Average 3 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 3 based on 1 users reviews)
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Date :
Anonymous submitted on 31-Oct-2011
Application Tested :
WB
Sample Species :
Hu
Sample :
293T cells
Amount used :
30μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
