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BrdU抗体

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091
Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

目录号 GTX128091

文献引用 13
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 ICC/IF, IHC-P, IHC-Fr, FACS, IHC

种属反应

 Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 BrdU 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to BrdU

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11168976

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091

GTX128091 IHC-P Image

BrdU antibody detects Bromodeoxyuridine (BrdU) in mouse BrdU treated intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU.
BrdU stained by BrdU antibody (GTX128091) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091

GTX128091 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4oC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

GTX128091 ICC/IF Image

BrdU antibody detects BrdU protein at nucleus and mitochondria by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BrdU stained by BrdU antibody (GTX128091) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

Application Reference

W Shi et al. Neuroscience 2023; S0306-4522 (23) : 00265-8 Hypoxic Postconditioning Promotes Angiogenesis After Ischemic Stroke
实验应用 : ICC/IF
Chen SY et al. Mol Ther Methods Clin Dev 2022; 26 : 157-168 Amelioration of experimental tendinopathy by lentiviral CD44 gene therapy targeting senescence-associated secretory phenotypes.
实验应用 : IHC-P
Lanzafame M et al. Nucleic Acids Res 2021; 49 (19) : 10911-10930 Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation.
Hattori Y et al. Biochem Pharmacol 2021; 186 : 114495 Attenuation of growth hormone production at the fetal stage is critical for dioxin-induced developmental disorder in rat offspring.
H?rdtner C et al. Basic Res Cardiol 2020; 115 (6) : 78 Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering.
实验应用 : IHC-Fr
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产品评论

BrdU antibody 目录号 GTX128091

Rating
( Average 4 based on 2 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-BrdU antibody used in IHC (Frozen sections) (IHC-Fr). GTX128091
Date : Anonymous submitted on 29-Dec-2015
Score :
Application Tested : IHC-Fr
Sample : zebrafish intestine mucosa tissue
Blocking : 5% normal goat serum, 0.5% Triton X-100 in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5%Triton X-100 in PBS/ 2 N HCl and 10 mM citric acid buffer
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : ICC/IF
Sample : U343 cells
Blocking : PBS + 0,5% BSA +0,5% Glycin, 20°C, 2Hr
Fixation : Methanol:Aceton (1:1)
Primary Antibody : 1:100, 4°C, 15Hr
Figure Note : U343 cells which were cultivated in DMEM medium + stimulation with 10% FCS for 24 and subsequent treatment with 10 μM BrdU for 18 h following fixation and staining.
我要评论产品
Application Reference
W Shi et al. Neuroscience 2023; S0306-4522 (23):00265-8 Hypoxic Postconditioning Promotes Angiogenesis After Ischemic Stroke
实验应用 : ICC/IF
Chen SY et al. Mol Ther Methods Clin Dev 2022; 26:157-168 Amelioration of experimental tendinopathy by lentiviral CD44 gene therapy targeting senescence-associated secretory phenotypes.
实验应用 : IHC-P
Lanzafame M et al. Nucleic Acids Res 2021; 49 (19):10911-10930 Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation.
Hattori Y et al. Biochem Pharmacol 2021; 186:114495 Attenuation of growth hormone production at the fetal stage is critical for dioxin-induced developmental disorder in rat offspring.
H?rdtner C et al. Basic Res Cardiol 2020; 115 (6):78 Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering.
实验应用 : IHC-Fr
Lazarchuk P et al. Mol Cell Biol 2020; (Epub) Mutual balance of histone deacetylases HDAC1, HDAC2, and the acetyl reader ATAD2 regulates the level of acetylation of histone H4 on nascent chromatin of human cells.
Liu G et al. Int J Biol Sci 2019; 15 (7):1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.
Guosheng Liu et al. Int J Biol Sci 2019; 15 (7):1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Ni YL et al. Chem Biol Interact 2018; 291:137-143 Teroxirone motivates apoptotic death in tumorspheres of human lung cancer cells.
实验应用 : IHC
Zhu B et al. Cell Mol Life Sci 2018; (Epub) Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.
实验应用 : IHC
Sun J et al. Brain Res 2013; 1491:225-35 WIN55, 212-2 promotes differentiation of oligodendrocyte precursor cells and improve remyelination through regulation of the phosphorylation level of the ERK 1/2 via cannabinoid receptor 1 after stroke-induced demyelination.
实验应用 : IHC
Stegeman H et al. Radiother Oncol 2013; 106 (3):383-9 Predictive value of hypoxia, proliferation and tyrosine kinase receptors for EGFR-inhibition and radiotherapy sensitivity in head and neck cancer models.
实验应用 : IHC-Fr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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