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C3 / C3b抗体 [C3], C-term

Anti-C3 / C3b antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101316
Anti-C3 / C3b antibody [C3], C-term used in Western Blot (WB). GTX101316
Anti-C3 / C3b antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101316
Anti-C3 / C3b antibody [C3], C-term used in Immunoprecipitation (IP). GTX101316
Anti-C3 / C3b antibody [C3], C-term used in Western Blot (WB). GTX101316

目录号 GTX101316

文献引用 5

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP, Immunosubtraction

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 C3 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Immunosubtraction Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

187 kDa. ( Note )

产品说明

The mouse reactivity of this antibody is based on mouse IHC-P testing and may be not suitable for mouse WB assay.

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240642

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

complement C3 , AHUS5 , ARMD9 , ASP , C3a , C3b , CPAMD1 , HEL-S-62p

细胞定位

Secreted

功能与背景

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-C3 / C3b antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101316

GTX101316 ICC/IF Image

C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-C3 / C3b antibody [C3], C-term used in Western Blot (WB). GTX101316

GTX101316 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GTX101316) diluted at 1:1000.

Anti-C3 / C3b antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101316

GTX101316 IHC-P Image

C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C3 antibody [C3], C-term (GTX101316) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C3 / C3b antibody [C3], C-term used in Immunoprecipitation (IP). GTX101316

GTX101316 IP Image

Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316).
Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-C3 / C3b antibody [C3], C-term used in Western Blot (WB). GTX101316

GTX101316 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Cao K et al. Alzheimers Res Ther 2019; 11 (1) : 35 Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation.
Gu矇guinou N et al. Int J Mol Sci 2019; 20 (7) Modulation of Iberian Ribbed Newt Complement Component C3 by Stressors Similar to those Encountered during a Stay Onboard the International Space Station.
Fukuoka Y et al. J Immunol 2013; 191 (4) : 1827-34 Human skin mast cells express complement factors C3 and C5.
实验应用 : WB, ICC/IF
种属反应 : Human
Arai S et al. Cell Rep 2013; 3 (4) : 1187-98 Obesity-associated autoantibody production requires AIM to retain the immunoglobulin M immune complex on follicular dendritic cells.
实验应用 : WB
种属反应 : Mouse
Kuroda Y et al. J Pharm Biomed Anal 2013; 76 : 29738 Improved capillary electrophoresis method for the analysis of carbohydrate-deficient transferrin in human serum, avoiding interference by complement C3.
实验应用 : Immunosubtraction
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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