产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888525
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
C9orf72-SMCR8 complex subunit , ALSFTD , DENNL72 , FTDALS , FTDALS1
细胞定位
Nucleus,Cytoplasm,Endosome,Lysosome,Secreted,Cell projection, dendrite
功能与背景
The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016]
数据库
研究领域
產品图片
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/templates/default/js/lightbox/images/loading.gif "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
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GTX635397 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635397 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635397 ICC/IF Image
C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 stained by C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX635397 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635397 IHC-P Image
C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.C9orf72 stained by C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635397 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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GTX635397 IHC-P Image
C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.C9orf72 stained by C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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|
GTX635397 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (GTX635397) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_43882_20200724_WB_shRNA_watermark_w_23061202_606.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_43882_20221209_WB_C_22121123_630.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_43882_20200715_ICC_IF_22072519_115.webp "Anti-C9orf72 antibody [GT779-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_45075_20230616_WB_23102401_899.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_43882_20220218_IHC-P_M_1_w_23061202_561.webp "Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_43882_20200529_WB_M_R_w_23061202_328.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_43882_20220218_IHC-P_M_w_23061202_615.webp "Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/small/GTX635397/GTX635397_43882_20230707_WB_B_23071300_453.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_43882_20200724_WB_shRNA_watermark_w_23061202_606.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_43882_20221209_WB_C_22121123_630.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_43882_20200715_ICC_IF_22072519_115.webp "Anti-C9orf72 antibody [GT779-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_45075_20230616_WB_23102401_899.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_43882_20220218_IHC-P_M_1_w_23061202_561.webp "Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_43882_20200529_WB_M_R_w_23061202_328.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_43882_20220218_IHC-P_M_w_23061202_615.webp "Anti-C9orf72 antibody [GT779-RB] used in IHC (Paraffin sections) (IHC-P). GTX635397")
![Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397](/upload/website/prouct_img/normal/GTX635397/GTX635397_43882_20230707_WB_B_23071300_453.webp "Anti-C9orf72 antibody [GT779-RB] used in Western Blot (WB). GTX635397")
