C9orf72抗体 [GT779]

Name: C9orf72抗体 [GT779]
Brand: GeneTex
SKU: GTX632041
Price: 4000.00 RMB
Rating: 4 (2 reviews)

Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041

Anti-C9orf72 antibody [GT779] used in Immunoprecipitation (IP). GTX632041

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

Anti-C9orf72 antibody [GT779] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632041

目录号 GTX632041

文献引用 14

宿主	Mouse
克隆	Monoclonal
克隆编号	GT779
同种型	IgG1
实验应用	WB, ICC/IF, IHC-P, IP
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX632041 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

阳性对照

mouse brain , rat brain

产品说明

Knockdown/Knockout validation was supported by customer review data

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2784546

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

C9orf72-SMCR8 complex subunit , ALSFTD , DENNL72 , FTDALS , FTDALS1

数据库

Gene ID: 203228 C9orf72
UniProt: Q96LT7 C9orf72

研究领域

產品图片

GTX632041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IP Image

Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 μg of C9orf72 antibody [GT779] (GTX632041).
Western blot analysis was performed using C9orf72 antibody [GT779] (GTX632041).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX632041 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632041 IHC-P Image

GTX632041 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632041 IHC-P Image

GTX632041 ICC/IF Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

Application Reference

F Limone et al. Sci Transl Med 2024; 16 (732) : eadg7895 Myeloid and lymphoid expression of C9orf72 regulates IL-17A signaling in mice

SE Salomonsson et al. Sci Rep 2024; 14 (1) : 828 Validated assays for the quantification of C9orf72 human pathology

实验应用 : WB

种属反应 : Human

He L et al. Cell Death Differ 2023; 30 (3) : 716-730 C9orf72 functions in the nucleus to regulate DNA damage repair.

ZS Chen et al. Nat Commun 2023; 14 (1) : 8420 Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD

实验应用 : WB

J?lg J et al. EMBO Rep 2023; 24 (8) : e55895 C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains.

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产品评论

C9orf72 antibody [GT779] 目录号 GTX632041

Rating

( Average 4 based on 2 users reviews)

实验应用

Western Blot(WB)		( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)		( Average 3 based on 1 users reviews)

Date : Anonymous submitted on 20-Oct-2022

Score :

Application Tested : IHC-P

Sample Species : Ms

Sample : Mouse brain tissue section, hippocampus, CA2-3 region

Blocking : Calf serum, 30°C, 1Hr

Fixation : PFA

Permeabilization /Antigen retrieval : TBS with 0.3% tritonX / pH9 antigen retireval buffer

Primary Antibody : 1:1000, 16°C, 4Hr

Date : Anonymous submitted on 18-Jan-2017

Score :

Application Tested : WB

Sample Species : Ms

Sample : Macrophages

Primary Antibody : 1:500, 4°C, 16Hr

条带说明 : Lane 1: wild type, Lane 2: heterozygous C9orf72 disruption, Lane 3: homozygous C9orf72 disruption

我要评论产品

Application Reference

F Limone et al. Sci Transl Med 2024; 16 (732):eadg7895 Myeloid and lymphoid expression of C9orf72 regulates IL-17A signaling in mice

SE Salomonsson et al. Sci Rep 2024; 14 (1):828 Validated assays for the quantification of C9orf72 human pathology

实验应用 : WB

种属反应 : Human

He L et al. Cell Death Differ 2023; 30 (3):716-730 C9orf72 functions in the nucleus to regulate DNA damage repair.

ZS Chen et al. Nat Commun 2023; 14 (1):8420 Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD

实验应用 : WB

Seidel M et al. Curr Res Neurobiol 2023; 5:100105 Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models.

J?lg J et al. EMBO Rep 2023; 24 (8):e55895 C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains.

Mira Seidel et al. Curr Opin Neurobiol 2023; 5 (2023):100105 Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models

实验应用 : WB

种属反应 : Human

Ghaffari LT et al. iScience 2023; 26 (6):106959 Differential response of C9orf72 transcripts following neuronal depolarization.

Pang W et al. iScience 2023; 26 (5):106579 C9ORF72 suppresses JAK-STAT mediated inflammation.

Liu Y et al. Mol Cell Neurosci 2022; 121:103756 Hyperactivity of Purkinje cell and motor deficits in C9orf72 knockout mice.

Bauer CS et al. Acta Neuropathol 2022; 144 (3):437-464 An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.

Liu Y et al. Nat Commun. 2021; 12 (1):847 Variant-selective stereopure oligonucleotides protect against pathologies associated with C9orf72-repeat expansion in preclinical models.

实验应用 : WB

种属反应 : Human

Laflamme C et al. Elife 2019; 8 Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

实验应用 : WB, ICC/IF, IP

种属反应 : Human

Carl Laflamme et al. bioRxiv 2018; (Epub) Implementation of an antibody characterization process: Application to the major ALS/FTD disease gene C9ORF72.

实验应用 : WB, ICC/IF, IP

种属反应 : Human, Mouse

SDS
PBS.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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C9orf72抗体 [GT779]

目录号 GTX632041

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品说明

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

数据库

研究领域

產品图片

GTX632041 WB Image

GTX632041 IP Image

GTX632041 WB Image

GTX632041 IHC-P Image

GTX632041 WB Image

GTX632041 IHC-P Image

GTX632041 IHC-P Image

GTX632041 IHC-P Image

GTX632041 IHC-P Image

GTX632041 WB Image

GTX632041 IHC-P Image

GTX632041 IHC-P Image

GTX632041 ICC/IF Image

文献引用

Application Reference

产品评论

C9orf72 antibody [GT779] 目录号 GTX632041

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