产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human CARD10.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
caspase recruitment domain family member 10 , BIMP1 , CARMA3
细胞定位
Cytoplasm
功能与背景
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]
数据库
研究领域
產品图片
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/templates/default/js/lightbox/images/loading.gif "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
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GTX638313 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (GTX638313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638313 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (GTX638313) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638313 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (GTX638313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638313 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (GTX638313) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/small/GTX638313/GTX638313_T-44970_20230303_WB_shRNA_watermark_23030717_854.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/small/GTX638313/GTX638313_45033_20230519_WB_R_liver_23053001_140.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/small/GTX638313/GTX638313_45033_20230505_WB_TPM_watermark_23050918_820.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/small/GTX638313/GTX638313_T-44970_20230331_WB_M_liver_23041023_207.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/normal/GTX638313/GTX638313_T-44970_20230303_WB_shRNA_watermark_23030717_854.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/normal/GTX638313/GTX638313_45033_20230519_WB_R_liver_23053001_140.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/normal/GTX638313/GTX638313_45033_20230505_WB_TPM_watermark_23050918_820.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
![Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313](/upload/website/prouct_img/normal/GTX638313/GTX638313_T-44970_20230331_WB_M_liver_23041023_207.webp "Anti-CARD10 antibody [HL2261] used in Western Blot (WB). GTX638313")
