Wild-type (WT) and CREBBP knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (GTX638201) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (GTX638201) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CBP antibody [HL2197] detects CBP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CBP stained by CBP antibody [HL2197] (GTX638201) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
CBP stained by CBP antibody [HL2197] (GTX638201) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CBP stained by CBP antibody [HL2197] (GTX638201) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (GTX638201) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.