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CBS抗体 [GT519]

KO/KD Overexpression
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777
Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

目录号 GTX628777

文献引用 3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT519

同种型

 IgG1

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CBS 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

61 kDa. ( Note )

阳性对照

rat liver , Raji , K562 , mouse liver , 293T , HeLa , CBS-transfected 293T

预测种属反应

Rabbit, Bovine, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888050

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cystathionine-beta-synthase , CBSL , HIP4

细胞定位

Cytoplasm , Nucleus

功能与背景

The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500.

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

KO/KD

Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

CBS antibody [GT519] detects CBS protein by Western blot analysis.
A. 30 μg Raji whole cell lysate/extract
B. 30 μg K562 whole cell lysate/extract
7.5 % SDS-PAGE
CBS antibody [GT519] (GTX628777) dilution: 1:1000

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777

GTX628777 IHC-P Image

CBS antibody [GT519] detects CBS protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
CBS antibody [GT519] (GTX628777) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777

GTX628777 IHC-P Image

CBS antibody [GT519] detects CBS protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CBS antibody [GT519] (GTX628777) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

CBS antibody [GT519] detects CBS protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
CBS antibody [GT519] (GTX628777) dilution: 1:2000

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

CBS antibody [GT519] detects CBS protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
7.5 % SDS-PAGE
CBS antibody [GT519] (GTX628777) dilution: 1:1000

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Zheng J et al. Cell Death Dis 2021; 12 (7) : 698 Sorafenib fails to trigger ferroptosis across a wide range of cancer cell lines.
实验应用 : WB
种属反应 : Human
Ahmad A et al. Int J Mol Med 2016; (Epub) Effect of endotoxemia in mice genetically deficient in cystathionine-γ-lyase, cystathionine-β-synthase or 3-mercaptopyruvate sulfurtransferase.
实验应用 : WB
种属反应 : Mouse
Pincini A et al. Cell Cycle 2013; 12 (15) : 2409-22 Identification of p130Cas/ErbB2-dependent invasive signatures in transformed mammary epithelial cells.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
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¥ 1700
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