产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse liver , 293T , HeLa , CBS-transfected 293T
预测种属反应
Rabbit, Bovine, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036461
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cystathionine-beta-synthase , CBSL , HIP4
细胞定位
Cytoplasm , Nucleus
功能与背景
The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX113400 WB Image
Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113400 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113400 IHC-P Image
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS(GTX113400) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113400 IP Image
CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CBS protein by 4 μg CBS antibody (GTX113400) 7.5 % SDS-PAGE The immunoprecipitated CBS protein was detected by CBS antibody (GTX113400) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX113400 WB Image
CBS antibody detects CBS protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113400 ICC/IF Image
CBS antibody detects CBS protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: CBS stained by CBS antibody (GTX113400) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX113400 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113400 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113400 IHC-P Image
CBS antibody detects CBS protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CBS stained by CBS antibody (GTX113400) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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