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CD10抗体 [N2C1], Internal

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680
Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680
Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680
Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680
Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680

目录号 GTX111680

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CD10 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

86 kDa. ( Note )

阳性对照

rat kidney

预测种属反应

Rabbit, Bovine, Dog, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human CD10. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10726415

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

membrane metalloendopeptidase , CALLA , CD10 , CMT2T , NEP , SCA43 , SFE

细胞定位

Cell membrane; Single-pass type II membrane protein

功能与背景

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680

GTX111680 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal (GTX111680) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680

GTX111680 WB Image

CD10 antibody [N2C1], Internal detects MME protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
7.5% SDS-PAGE
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680

GTX111680 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal (GTX111680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680

GTX111680 IHC-P Image

CD10 antibody [N2C1], Internal detects CD10 protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680

GTX111680 IHC-P Image

CD10 antibody [N2C1], Internal detects MME protein at cytosol and membrane on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Yap XL et al. Membranes (Basel) 2021; 11 (8) Detection of Prostate Cancer via IR Spectroscopic Analysis of Urinary Extracellular Vesicles: A Pilot Study.
实验应用 : WB
种属反应 : Human
Fukui K et al. Neurosci Res 2017; (Epub) Rebamipide reduces amyloid-β 1-42 (Aβ42) production and ameliorates Aβ43-lowered cell viability in cultured SH-SY5Y human neuroblastoma cells.
实验应用 : WB
种属反应 : Human
Wang S et al. J Biol Chem 2013; 288 (36) : 26265-74 Progesterone receptor A stability is mediated by glycogen synthase kinase-3β in the Brca1-deficient mammary gland.
实验应用 : WB
种属反应 : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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