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CD133抗体 [C1C2], Internal

Orthogonal Overexpression
Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567
Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567
Anti-CD133 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100567
Anti-CD133 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100567
Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567

目录号 GTX100567

文献引用 10
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, FACS

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CD133 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

97 kDa. ( Note )

阳性对照

3xFlag-human CD133-transfected 293T cells

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human CD133. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240577

注意事项

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

相关讯息

别名

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

细胞定位

Apical cell membrane,Endoplasmic reticulum

功能与背景

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

数据库

研究领域

產品图片

Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567

GTX100567 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [C1C2], Internal (GTX100567) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29760280

Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567

GTX100567 WB Image

Overexpression

CD133 antibody [C1C2], Internal detects CD133 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells
7.5 % SDS-PAGE
CD133 antibody [C1C2], Internal (GTX100567) dilution: 1:5000

Anti-CD133 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100567

GTX100567 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using CD133(GTX100567) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD133 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100567

GTX100567 IHC-P Image

CD133 antibody [C1C2], Internal detects CD133 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CD133 stained by CD133 antibody [C1C2], Internal (GTX100567) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567

GTX100567 WB Image

Orthogonal

The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384

文献引用

Application Reference

YF Tsai et al. Cancer Cell Int 2023; RAD51 is a poor prognostic marker and a potential therapeutic target for oral squamous cell carcinoma
实验应用 : WB
Cardile A et al. Antioxidants (Basel) 2023; 12 (7) Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers.
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4) : 866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
实验应用 : WB
种属反应 : Human
Jao TM et al. Cancer Lett 2021; 499 : 290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
实验应用 : WB
种属反应 : Human
Wu S et al. Cancers (Basel) 2020; 12 (12) Establishment of an Immunocompetent Metastasis Rat Model with Hepatocyte Cancer Stem Cells.
实验应用 : ICC/IF
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产品评论

CD133 antibody [C1C2], Internal 目录号 GTX100567

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
条带说明 : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate
Anti-CD133 antibody [C1C2], Internal used in Flow cytometry (FACS). GTX100567
Date : Anonymous submitted on 07-Jan-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : A549 cells
Primary Antibody : 1:200, 4°C, 0.25Hr
我要评论产品
Application Reference
YF Tsai et al. Cancer Cell Int 2023; RAD51 is a poor prognostic marker and a potential therapeutic target for oral squamous cell carcinoma
实验应用 : WB
Cardile A et al. Antioxidants (Basel) 2023; 12 (7) Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers.
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
实验应用 : WB
种属反应 : Human
Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
实验应用 : WB
种属反应 : Human
Wu S et al. Cancers (Basel) 2020; 12 (12) Establishment of an Immunocompetent Metastasis Rat Model with Hepatocyte Cancer Stem Cells.
实验应用 : ICC/IF
Raineri A et al. Int J Mol Sci 2019; 20 (23) Onconase Restores Cytotoxicity in Dabrafenib-Resistant A375 Human Melanoma Cells and Affects Cell Migration, Invasion and Colony Formation Capability.
Wu HY et al. Cancer Lett 2019; 456:13-22 p21-Activated kinase 3 promotes cancer stem cell phenotypes through activating the Akt-GSK3β-β-catenin signaling pathway in pancreatic cancer cells.
实验应用 : WB
种属反应 : Human
Ma L et al. PLoS One 2017; 12 (11):e0188665 Suppression of cancer stemness by upregulating Ligand-of-Numb protein X1 in colorectal carcinoma.
实验应用 : WB
种属反应 : Human
Huang WT et al. Eur J Pharm Biopharm 2016; (Epub) dual drug-loaded biofunctionalized amphiphilic chitosan nanoparticles: enhanced synergy between * and demethoxycurcumin against multidrug-resistant stem-like lung cancer cells.
Qiu HO et al. Med Sci Monit 2016; 22:1146-51 Identification and Characterization of CD133pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line.
实验应用 : FACS
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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