产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human CD133 Extracellular domain.
预测种属反应
Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human CD133. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910041
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4
细胞定位
Apical cell membrane,Endoplasmic reticulum
功能与背景
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
数据库
研究领域
產品图片
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/templates/default/js/lightbox/images/loading.gif "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
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GTX636673 IHC-P Image
CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.CD133 stained by CD133 antibody [HL1271] (GTX636673) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636673 IHC-P Image
CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.CD133 stained by CD133 antibody [HL1271] (GTX636673) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636673 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636673 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_M_22122821_353.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_R_22122821_743.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_44599_20220225_WB_w_23061202_503.webp "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_T-44536_20220121_WB_B_w_23061202_855.webp "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_M_22122821_353.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_R_22122821_743.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20220225_WB_w_23061202_503.webp "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_T-44536_20220121_WB_B_w_23061202_855.webp "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
