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CD16 + CD32抗体 [2.4G2] (PE-Cy7)

Anti-CD16 + CD32 antibody [2.4G2] (PE-Cy7) used in Flow cytometry (FACS). GTX01453-10

目录号 GTX01453-10

文献引用 283

宿主

 Rat

克隆

 Monoclonal

克隆编号

 2.4G2

同种型

 IgG2b

实验应用

 FACS

种属反应

 Mouse
产品包装
100 μg (¥4000)
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产品说明

摘要

The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
FACS Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

37 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

纯化方式

Purified by affinity chromatography
From tissue culture supernatant

偶联

Phycoerythrin-Cyanine7 (PE-Cy7)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Fc receptor, IgG, low affinity IIb , Fc receptor, IgG, low affinity III , AI528646 , CD32 , F630109E10Rik , Fc[g]RII , FcgRII , Fcgr2 , Fcgr2a , Fcr-2 , Fcr-3 , Ly-17 , Ly-m20 , LyM-1 , Lym-1 , fcRII , CD16

细胞定位

Cell membrane; Single-pass type I membrane protein

数据库

研究领域

產品图片

Anti-CD16 + CD32 antibody [2.4G2] (PE-Cy7) used in Flow cytometry (FACS). GTX01453-10

GTX01453-10 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01453-10 CD16 + CD32 antibody [2.4G2] (PE-Cy7).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

文献引用

Clone Reference

Sitnik S et al. Mol Ther Oncolytics 2020; 17:190-204 PD-1 IC Inhibition Synergistically Improves Influenza A Virus-Mediated Oncolysis of Metastatic Pulmonary Melanoma.
Kallis MP et al. J Transl Med 2020; 18 (1):183 Pharmacological prevention of surgery-accelerated metastasis in an animal model of osteosarcoma.
Becerril-Garc?a MA et al. Front Immunol 2020; 11:744 Langerhans Cells From Mice at Birth Express Endocytic- and Pattern Recognition-Receptors, Migrate to Draining Lymph Nodes Ferrying Antigen and Activate Neonatal T Cells in vivo.
Patta I et al. Nucleic Acids Res 2020; 48 (11):5873-5890 Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.
Wilhelmson AS et al. Front Immunol 2020; 11:1342 Androgen Receptors in Epithelial Cells Regulate Thymopoiesis and Recent Thymic Emigrants in Male Mice.
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Clone Reference
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