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CD16 + CD32抗体 [2.4G2] (PE)

Anti-CD16 + CD32 antibody [2.4G2] (PE) used in Flow cytometry (FACS). GTX01453-08

目录号 GTX01453-08

文献引用 283

宿主

 Rat

克隆

 Monoclonal

克隆编号

 2.4G2

同种型

 IgG2b

实验应用

 FACS

种属反应

 Mouse
产品包装
100 μg (¥3300)
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产品说明

摘要

The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
FACS Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

37 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

纯化方式

Purified by affinity chromatography
From tissue culture supernatant

偶联

Phycoerythrin (PE)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Fc receptor, IgG, low affinity IIb , Fc receptor, IgG, low affinity III , AI528646 , CD32 , F630109E10Rik , Fc[g]RII , FcgRII , Fcgr2 , Fcgr2a , Fcr-2 , Fcr-3 , Ly-17 , Ly-m20 , LyM-1 , Lym-1 , fcRII , CD16

细胞定位

Cell membrane; Single-pass type I membrane protein

数据库

研究领域

產品图片

Anti-CD16 + CD32 antibody [2.4G2] (PE) used in Flow cytometry (FACS). GTX01453-08

GTX01453-08 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01453-08 CD16 + CD32 antibody [2.4G2] (PE).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.125 μg (5 μl)

文献引用

Clone Reference

Sitnik S et al. Mol Ther Oncolytics 2020; 17:190-204 PD-1 IC Inhibition Synergistically Improves Influenza A Virus-Mediated Oncolysis of Metastatic Pulmonary Melanoma.
Kallis MP et al. J Transl Med 2020; 18 (1):183 Pharmacological prevention of surgery-accelerated metastasis in an animal model of osteosarcoma.
Becerril-Garc?a MA et al. Front Immunol 2020; 11:744 Langerhans Cells From Mice at Birth Express Endocytic- and Pattern Recognition-Receptors, Migrate to Draining Lymph Nodes Ferrying Antigen and Activate Neonatal T Cells in vivo.
Patta I et al. Nucleic Acids Res 2020; 48 (11):5873-5890 Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.
Wilhelmson AS et al. Front Immunol 2020; 11:1342 Androgen Receptors in Epithelial Cells Regulate Thymopoiesis and Recent Thymic Emigrants in Male Mice.
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Clone Reference
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