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CD161抗体 [HP-3G10] (APC)

Anti-CD161 antibody [HP-3G10] (APC) used in Flow cytometry (FACS). GTX01454-07

目录号 GTX01454-07

文献引用 174

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 HP-3G10

同种型

 IgG1

实验应用

 FACS

种属反应

 Human, Baboon, Chimpanzee, Rhesus Monkey
产品包装
100 test (¥3500)
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产品说明

摘要

The HP-3G10 antibody is specific for human CD161, also known as NKR-P1A, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. CD161 exists as a homodimer which is prominently expressed on natural killer (NK) and NKT cells, where it is proposed to regulate the function of both cell types. CD161 is also found on T cell subsets, including T regulatory cells (Tregs), memory/effector CD4+ T cells, and CD8+ T cells. Th17 cells have been demonstrated to co-express CD161, as surface IL-17A+ cells are contained within the CD161+ fraction of CD4 T cells, so that CD161 (in combination with CCR6) is often used as a marker for enrichment of Th17 cells.The HP-3G10 antibody may be used for flow cytometric analysis of CD161 on NK and NKT cells, as well as on various T cell subsets. The antibody is also reported to be cross-reactive with Baboon, Chimpanzee and Rhesus CD161.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

25 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

浓度

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

纯化方式

Purified by affinity chromatography
From tissue culture supernatant

偶联

Allophycocyanin (APC)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

killer cell lectin like receptor B1 , CD161 , CLEC5B , KLRB1 , NKR , NKRP1 , NKRP1A , hNKRP1A

细胞定位

Membrane

功能与背景

Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-CD161 antibody [HP-3G10] (APC) used in Flow cytometry (FACS). GTX01454-07

GTX01454-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01454-07 CD161 antibody [HP-3G10] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

文献引用

Clone Reference

Salio M et al. Proc Natl Acad Sci U S A 2020; 117 (19):10465-10475 Ligand-dependent downregulation of MR1 cell surface expression.
Sharma M et al. Front Immunol 2020; 11:1136 Mucosal-Associated Invariant T Cells Develop an Innate-Like Transcriptomic Program in Anti-mycobacterial Responses.
Schulte-Schrepping J et al. Cell 2020; Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment.
Rodriguez L et al. Cell Rep Med 2020; 1 (5):100078 Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19.
Sztein MB et al. Sci Rep 2020; 10 (1):13581 Salmonella enterica serovar Typhi exposure elicits ex vivo cell-type-specific epigenetic changes in human gut cells.
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Clone Reference
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