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CD20抗体 [C2C3], C-term

Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209
Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209
Anti-CD20 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100209
Anti-CD20 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100209
Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

目录号 GTX100209

文献引用 1
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CD20 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

33 kDa. ( Note )

阳性对照

K562 , THP-1

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human CD20. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1080937

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

membrane spanning 4-domains A1 , B1 , Bp35 , CD20 , CVID5 , LEU-16 , MS4A2 , S7

细胞定位

Membrane

功能与背景

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

GTX100209 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted withCD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

GTX100209 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD20 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100209

GTX100209 ICC/IF Image

CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD20 protein stained by CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CD20 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100209

GTX100209 IHC-P Image

CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Green: CD20 stained by CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

GTX100209 WB Image

Sample (30 ug of whole cell lysate)
A: K562
B: THP-1
12% SDS PAGE
GTX100209 diluted at 1:10000

文献引用

Application Reference

Atif SM et al. JCI Insight 2019; 5 Protective role of B cells in sterile particulate-induced lung injury.
实验应用 : IHC-P
种属反应 : Human
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产品评论

CD20 antibody [C2C3], C-term 目录号 GTX100209

Rating
( Average 4 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-CD20 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100209
Date : Anonymous submitted on 04-May-2023
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human ccRCC (Clear cell renal cell carcinoma) tissue section
Blocking : Donkey serum 5% in TBS, 22°C, 1-2Hr
Permeabilization /Antigen retrieval : EDTA pH 8.0
Primary Antibody : 1:100, 4°C, 12Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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