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CD27抗体 [O323] (APC)

Anti-CD27 antibody [O323] (APC) used in Flow cytometry (FACS). GTX01457-07

目录号 GTX01457-07

文献引用 416

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 O323

同种型

 IgG1

实验应用

 FACS

种属反应

 Human, Baboon, Cynomolgus monkey, Rhesus Monkey
产品包装
100 test (¥2900)
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产品说明

摘要

The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of ≥ 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD1d, IL-10 and TGF-beta. The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

29 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

浓度

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

纯化方式

Purified by affinity chromatography
From tissue culture supernatant

偶联

Allophycocyanin (APC)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CD27 molecule , CD27 , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55

细胞定位

Membrane

功能与背景

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-CD27 antibody [O323] (APC) used in Flow cytometry (FACS). GTX01457-07

GTX01457-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01457-07 CD27 antibody [O323] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

文献引用

Clone Reference

Bowyer G et al. J Exp Med 2020; 217 (7) Reduced Ebola vaccine responses in CMV+ young adults is associated with expansion of CD57+KLRG1+ T cells.
Badura R et al. Front Cell Infect Microbiol 2020; 10:347 Early ART in Acute HIV-1 Infection: Impact on the B-Cell Compartment.
Rivera-Correa J et al. PLoS Negl Trop Dis 2020; 14 (7):e0008466 Atypical memory B-cells and autoantibodies correlate with anemia during Plasmodium vivax complicated infections.
Kreye J et al. bioRxiv 2020; A SARS-CoV-2 neutralizing antibody protects from lung pathology in a COVID-19 hamster model.
Kriegsmann K et al. Cancers (Basel) 2020; 12 (8) Comparison of NGS and MFC Methods: Key Metrics in Multiple Myeloma MRD Assessment.
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Clone Reference
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