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CD31抗体

Comparison
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Anti-CD31 antibody used in Western Blot (WB). GTX130274
Anti-CD31 antibody used in Western Blot (WB). GTX130274
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

目录号 GTX130274

文献引用 13
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, IHC-Fr

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CD31 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

81 kDa. ( Note )

阳性对照

mouse spleen

预测种属反应

Rat(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Synthetic peptide encompassing a sequence within the Extracellular domain of mouse CD31. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886228

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

platelet/endothelial cell adhesion molecule 1 , C85791 , Cd31 , PECAM-1 , Pecam

细胞定位

Membrane; Single-pass type I membrane protein

功能与背景

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation (By similarity). Induces susceptibility to atherosclerosis.

数据库

研究领域

產品图片

Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

GTX130274 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse artery.
CD31 stained by CD31 antibody (GTX130274) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD31 antibody used in Western Blot (WB). GTX130274

GTX130274 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CD31 antibody (GTX130274) diluted at 1:500 and competitor's antibody (ab124432) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD31 antibody used in Western Blot (WB). GTX130274

GTX130274 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD31 antibody (GTX130274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

GTX130274 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
CD31 stained by CD31 antibody (GTX130274) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274

GTX130274 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Green: CD31 stained by CD31 antibody (GTX130274) diluted at 1:250.
Red: beta Actin, a Cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

PS Sung et al. EMBO Mol Med 2023; : e16351 Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc
种属反应 : Mouse
Nishikawa S et al. Diagnostics (Basel) 2022; 12 (10) Characteristic of Concurrent Uterine Lipoleiomyoma and Hemangioma by Algorithm of Candidate Biomarkers for Uterine Mesenchymal Tumor.
种属反应 : Human
Gu X et al. Acta Biomater 2022; (Epub) Integrated polycaprolactone microsphere-based scaffolds with biomimetic hierarchy and tunable vascularization for osteochondral repair.
Meng-Jin Lin et al. Int J Mol Sci 2021; 22 (12) Sustained Release of Insulin-Like Growth Factor-1 from Bombyx mori L. Silk Fibroin Delivery for Diabetic Wound Therapy.
实验应用 : IHC-P
种属反应 : Mouse
Lin MJ et al. Pharmaceutics 2021; 13 (9) An Insulin-like Growth Factor-1 Conjugated Bombyx mori Silk Fibroin Film for Diabetic Wound Healing: Fabrication, Physicochemical Property Characterization, and Dosage Optimization In Vitro and In Vivo.
实验应用 : IHC-P
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产品评论

CD31 antibody 目录号 GTX130274

Rating
( Average 4 based on 2 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 2 users reviews)
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Date : Anonymous submitted on 18-Apr-2023
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse skin tissue
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 10mM Sodium Citrate Buffer (pH6.0)
Primary Antibody : 1:100, 25°C, 16Hr
Anti-CD31 antibody used in IHC (Paraffin sections) (IHC-P). GTX130274
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse placenta
Blocking : 10% normal serum with 1% BSA, 25°C, 1Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate Buffer pH 6.0
Primary Antibody : 1:200, 25°C, 1Hr
我要评论产品
Application Reference
PS Sung et al. EMBO Mol Med 2023;:e16351 Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc
种属反应 : Mouse
Nishikawa S et al. Diagnostics (Basel) 2022; 12 (10) Characteristic of Concurrent Uterine Lipoleiomyoma and Hemangioma by Algorithm of Candidate Biomarkers for Uterine Mesenchymal Tumor.
种属反应 : Human
Gu X et al. Acta Biomater 2022; (Epub) Integrated polycaprolactone microsphere-based scaffolds with biomimetic hierarchy and tunable vascularization for osteochondral repair.
Meng-Jin Lin et al. Int J Mol Sci 2021; 22 (12) Sustained Release of Insulin-Like Growth Factor-1 from Bombyx mori L. Silk Fibroin Delivery for Diabetic Wound Therapy.
实验应用 : IHC-P
种属反应 : Mouse
Hu JL et al. Cell Transplant 2021; 30:9636897211041966 Impact of Injection Frequency of Adipose-Derived Stem Cells on Allogeneic Skin Graft Survival Outcomes in Mice.
实验应用 : IHC-P
种属反应 : Mouse
Lin MJ et al. Pharmaceutics 2021; 13 (9) An Insulin-like Growth Factor-1 Conjugated Bombyx mori Silk Fibroin Film for Diabetic Wound Healing: Fabrication, Physicochemical Property Characterization, and Dosage Optimization In Vitro and In Vivo.
实验应用 : IHC-P
Wang HK et al. Diab Vasc Dis Res 2021; 18 (1):1479164121990641 Hyperglycemia compromises the ischemia-provoked dedifferentiation of cerebral pericytes through p21-SOX2 signaling in high-fat diet-induced murine model.
实验应用 : IHC-Fr
种属反应 : Mouse
Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.
实验应用 : IHC-Fr
种属反应 : Mouse
Yeh LC et al. Int J Mol Sci 2020; 21 (5) The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia.
Deng Q et al. Stem Cell Res Ther 2020; 11 (1):143 PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.
Zhu L et al. Carbohydr Polym 2020; 240:116271 Effect of Chitosan oligosaccharides on ischemic symptom and gut microbiota disbalance in mice with hindlimb ischemia.
实验应用 : IHC-P
种属反应 : Mouse
Zheng J et al. J Ethnopharmacol 2019;:112330 BuZangTongLuo decoction improved hindlimb ischemia by activating angiogenesis and regulating gut microbiota in diabetic mice.
实验应用 : IHC-P
种属反应 : Mouse
Ishii N et al. Biol Reprod 2019; Ascorbic acid and CoQ10 ameliorate the reproductive ability of SOD1-deficient female mice.
实验应用 : IHC-P
种属反应 : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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