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CD44抗体 [GT462]

KO/KD Orthogonal IP/MS
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Flow cytometry (FACS). GTX628895
Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895
Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895

目录号 GTX628895

文献引用 5
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT462

同种型

 IgG2b

实验应用

 WB, IHC-P, FACS, IP

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

82 kDa. ( Note )

阳性对照

A431 , MDA-MB-231(24 μg/ml Tunicamycin treatment for 16 hr) , HeLa(12 μg/ml Tunicamycin treatment for 48 hr)

产品说明

This antibody was raised against human CD44 Extracellular domain.
IP/MS validation was supported by references (PMID:30377425)

预测种属反应

Dog(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888071

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

细胞定位

Membrane

功能与背景

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

KO/KD

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:500.

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.
Multiple bands were observed 130~180 kDa. It is possibly due to alternative splicing.
Reference: PMID: 20962267

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

KO/KD

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.(br)Reference:PMID: 10050880

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [GT462] used in Flow cytometry (FACS). GTX628895

GTX628895 FACS Image

CD44 antibody detects CD44 protein by flow cytometry analysis.
Sample: HL-60 cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Black: Isotype control dilution: 1:100.
Green: CD44 antibody (GTX628895) dilution: 1:100.

Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895

GTX628895 IHC-P Image

CD44 antibody [GT462] detects CD44 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
CD44 antibody [GT462] (GTX628895) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895

GTX628895 IHC-P Image

CD44 antibody [GT462] detects CD44 protein at cell membrane by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
CD44 stained by CD44 antibody [GT462] (GTX628895) diluted at 1:200.
Antigen Retrieval: EDTA buffer, 20 min

文献引用

Application Reference

Justyna Mazurkiewicz et al. Cell Commun Signal 2022; 20 (1) : 63 Melanoma cells with diverse invasive potential differentially induce the activation of normal human fibroblasts.
实验应用 : WB
种属反应 : Human
Xu H et al. Tissue Eng Part A 2021; Stem cell-seeded 3D-printed scaffolds combined with self-assembling peptides for bone defect repair.
实验应用 : FACS
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
实验应用 : IP
种属反应 : Human
Kim MC et al. BMC Cancer 2018; 18 (1) : 819 Hypoxia promotes acquisition of aggressive phenotypes in human malignant mesothelioma.
实验应用 : FACS
种属反应 : Human
Valarmathi MT et al. Tissue Eng Part A 2017; (Epub) Functional Tissue-Engineering: A Prevascularized Cardiac Muscle Construct for Validating hMSCs Engraftment Potential in Vitro.
实验应用 : FACS
种属反应 : Human
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产品评论

CD44 antibody [GT462] 目录号 GTX628895

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : breast cancer tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:200, 4°C, 16Hr
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PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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