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CD44抗体 [HL1650]

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231
Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231
Anti-CD44 antibody [HL1650] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

目录号 GTX637231

宿主

Rabbit

克隆

Monoclonal

克隆编号

HL1650

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

82 kDa. ( Note )

产品说明

This antibody was raised against human CD44 Extracellular domain.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

细胞定位

Cell membrane

功能与背景

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231

GTX637231 IHC-P Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog lung.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231

GTX637231 IHC-P Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231

GTX637231 IHC-P Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD44 antibody [HL1650] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637231

GTX637231 ICC/IF Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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