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CD44抗体

KO/KD Comparison Orthogonal IP/MS
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111
Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111
Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111
Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Anti-CD44 antibody used in Live cell imaging (LCI). GTX102111
Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111
Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111

目录号 GTX102111

文献引用 42
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IP, LCI

种属反应

 Human, Mouse, Rat, Rabbit
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 CD44 的产品
产品说明

摘要

CD44 is an 82kDa multifunctional transmembrane glycoprotein involved in cell migration, proliferation, differentiation, and signaling pathways that mediate cell survival. It is involved in many malignancies as well as being recognized as a cancer stem cell marker. Importantly, it is expressed in leukocytes and is a marker of leukemia-initiating cells, being found to be critical in the pathogenesis of T cell acute lymphoblastic leukemia.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
LCI Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

82 kDa. ( Note )

阳性对照

MDA-MB-231(24 μg/ml Tunicamycin treatment for 16 hr) , HeLa(12 μg/ml Tunicamycin treatment for 48 hr)

产品说明

IP/MS validation was supported by references (PMID:30377381)

预测种属反应

Dog(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240596

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

细胞定位

Membrane

功能与背景

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

KO/KD

Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody (GTX102111) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

KO/KD Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.
Reference: PMID: 10050880

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.
Reference: PMID: 10050880

Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111

GTX102111 IHC-P Image

CD44 antibody detects CD44 protein at membrane and cytoplasm on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma .
CD44 antibody (GTX102111) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111

GTX102111 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44(GTX102111) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111

GTX102111 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44(GTX102111) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111

GTX102111 ICC/IF Image

CD44 antibody detects CD44 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD44 stained by CD44 antibody (GTX102111) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111

GTX102111 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse brain, using CD44(GTX102111) antibody at 1:500 dilution.

Anti-CD44 antibody used in IHC (Paraffin sections) (IHC-P). GTX102111

GTX102111 IHC-P Image

CD44 antibody detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CD44 stained by CD44 antibody (GTX102111) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX102111) diluted at 1:45000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2015.PMID: 26136074

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2018.PMID: 29955155

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2015.PMID: 26136074

Anti-CD44 antibody used in Western Blot (WB). GTX102111

GTX102111 WB Image

KO/KD

The data was published in the journal Cell Death Dis in 2015.PMID: 26136074

Anti-CD44 antibody used in Live cell imaging (LCI). GTX102111

GTX102111 LCI Image

The data was published in the 2021 in Commun Biol. PMID: 33637851

Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111

GTX102111 ICC/IF Image

The data was published in the 2021 in Commun Biol. PMID: 33637851

Anti-CD44 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102111

GTX102111 ICC/IF Image

The data was published in the journal Oncotarget in 2016. PMID: 27487131

文献引用

Application Reference

Wang T et al. Mol Oncol 2024; 18 (1) : 44-61 SETD2 loss in renal epithelial cells drives epithelial-to-mesenchymal transition in a TGF-β-independent manner.
? ?en et al. ACS Appl Mater Interfaces 2023; 15 (50) : 58260-58273 Microglia Polarization and Antiglioma Effects Fostered by Dual Cell Membrane-Coated Doxorubicin-Loaded Hexagonal Boron Nitride Nanoflakes
Chu PY et al. Cell Biosci 2022; 12 (1) : 124 IFITM3 promotes malignant progression, cancer stemness and chemoresistance of gastric cancer by targeting MET/AKT/FOXO3/c-MYC axis.
实验应用 : WB
种属反应 : Human
Vadhan A et al. Biomedicines 2022; 10 (10) CD44 Promotes Breast Cancer Metastasis through AKT-Mediated Downregulation of Nuclear FOXA2.
Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
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产品评论

CD44 antibody 目录号 GTX102111

Rating
( Average 4.5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate
Anti-CD44 antibody used in Western Blot (WB). GTX102111
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCM1 cells
Amount used : 75μg
Blocking : 5% skim milk, 37°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
我要评论产品
Application Reference
Wang T et al. Mol Oncol 2024; 18 (1):44-61 SETD2 loss in renal epithelial cells drives epithelial-to-mesenchymal transition in a TGF-β-independent manner.
? ?en et al. ACS Appl Mater Interfaces 2023; 15 (50):58260-58273 Microglia Polarization and Antiglioma Effects Fostered by Dual Cell Membrane-Coated Doxorubicin-Loaded Hexagonal Boron Nitride Nanoflakes
Chu PY et al. Cell Biosci 2022; 12 (1):124 IFITM3 promotes malignant progression, cancer stemness and chemoresistance of gastric cancer by targeting MET/AKT/FOXO3/c-MYC axis.
实验应用 : WB
种属反应 : Human
Vadhan A et al. Biomedicines 2022; 10 (10) CD44 Promotes Breast Cancer Metastasis through AKT-Mediated Downregulation of Nuclear FOXA2.
Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
Howley BV et al. Oncogene 2022; (Epub) The ubiquitin E3 ligase ARIH1 regulates hnRNP E1 protein stability, EMT and breast cancer progression.
实验应用 : WB
种属反应 : Human
Young MJ et al. J Biomed Sci 2022; 29 (1):3 Estradiol-mediated inhibition of Sp1 decreases miR-3194-5p expression to enhance CD44 expression during lung cancer progression.
实验应用 : WB, IHC-P
种属反应 : Human
Cristina Mir et al. Cancers (Basel) 2021; 13 (19) SDCBP Modulates Stemness and Chemoresistance in Head and Neck Squamous Cell Carcinoma through Src Activation.
实验应用 : WB
种属反应 : Human
Yi-An Chen et al. Sci Rep 2021; 11 (1):22430 Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands.
实验应用 : ICC/IF, IHC-Fr
种属反应 : Human
Offeddu GS et al. Commun Biol 2021; 4 (1):255 The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination.
实验应用 : WB
种属反应 : Human
Yeh BW et al. Toxicol Appl Pharmacol 2020; 402:115129 The protoapigenone analog WYC0209 targets CD133+ cells: A potential adjuvant agent against cancer stem cells in urothelial cancer therapy.
实验应用 : IHC-P
种属反应 : Human
Teiichiro Aoyagi et al. Scientific Research 2020; 10:ID:98104,7 Basal Cell Hyperplasia Observed at Biopsy Specimens after HIFU Therapy: Implication of Stemness for Organ Regeneration.
实验应用 : IHC-P
种属反应 : Human
Li G et al. Front Mol Neurosci 2019; 12:212 Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations.
实验应用 : ICC/IF
种属反应 : Human
Lai HT et al. J Cell Physiol 2019; MicroRNA-203 diminishes the stemness of human colon cancer cells by suppressing GATA6 expression.
Chung SY et al. J Cell Physiol 2019; (Epub) Elucidation of the mechanism underlying CD44v6-induced transformation of IEC-6 normal intestinal epithelial cells.
实验应用 : WB
种属反应 : Human
LaRocca TJ et al. Neurobiol Dis 2019;:104514 TDP-43 knockdown causes innate immune activation via protein kinase R in astrocytes.
实验应用 : WB
种属反应 : Rat
Lin SC et al. Biochem Cell Biol 2019; (Epub) Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
实验应用 : WB
种属反应 : Human
Su YH et al. Oncotarget 2018; 9 (29):20490-20507 A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids.
Nan JN et al. Korean J Intern Med 2018; (Epub) β-Catenin expression is associated with cell invasiveness in pancreatic cancer.
实验应用 : WB
种属反应 : Human
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
实验应用 : IP
种属反应 : Human
Lin YH et al. Endocr Relat Cancer 2018; 25 (12):967-979 Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.
实验应用 : WB
种属反应 : Human
Chang CC et al. Sci Rep 2018; 8 (1):9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.
实验应用 : WB
种属反应 : Human
Aoyagi T et al. JCT 2018; 9:No.3 Basal Cell Proliferation Induced by Chlormadinone Acetate Suggests Stem Cell Transformation of Prostatic Cells.
实验应用 : IHC-P
种属反应 : Human
Xiong J et al. Mol Cell 2018; 69 (4):689-698.e7 A Metabolic Basis for Endothelial-to-Mesenchymal Transition.
实验应用 : WB
种属反应 : Human
Chang YT et al. Sci Rep 2017; 7:42795 REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer.
Lin PY et al. Am J Transl Res 2017; 9 (8):3827-3841 Therapeutic effect of * and * on ameliorating high-cholesterol diet-induced fatty liver disease, fibrosis and inflammation in rabbit.
实验应用 : IHC-P
种属反应 : Rabbit
Ni YL et al. Apoptosis 2017; (Epub) A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
实验应用 : WB
种属反应 : Human
Jiang X et al. Mol Med Rep 2017; 15 (3):1123-1134 Combination Twist1 and CA15-3 in axillary lymph nodes for breast cancer prognosis.
实验应用 : IHC-P
种属反应 : Human
Huang D et al. Mol Pharm 2017; (Epub) Hyaluronic Acid Coated Albumin Nanoparticles for Targeted Peptide Delivery to the Retina.
种属反应 : Human
Sun SJ et al. Oncotarget 2016; 7 (34):55572-55584 Integrin β3 and CD44 levels determine the effects of the OPN-a splicing variant on lung cancer cell growth.
实验应用 : ICC/IF
种属反应 : Human
Lin CW et al. Acta Biomater 2016; cd44-specific nanoparticles for redox-triggered reactive oxygen species production and * release.
实验应用 : WB
种属反应 : Human
Zhao B et al. J Proteomics 2015; 112:38-52 Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.
实验应用 : WB
种属反应 : Human
Zhang Y et al. Oncotarget 2015; 6 (12):9999-10015 aspirin counteracts cancer stem cell features, desmoplasia and * resistance in pancreatic cancer.
实验应用 : IHC-P
种属反应 : Human
Ju SY et al. Cell Death Dis 2015; 6 (Epub):e1801 identification of * as a novel therapeutic agent that targets human colon cancer stem cells.
实验应用 : WB
种属反应 : Human
N Yokdang et al. Oncogene 2015; 35 (22):2932-47 LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells[Epub ahead]
实验应用 : WB
种属反应 : Human
Tay LX et al. Int J Med Sci 2014; 11 (1):24-33 Differential protein expression between chondrogenic differentiated MSCs, undifferentiated MSCs and adult chondrocytes derived from Oryctolagus cuniculus in vitro.
实验应用 : ICC/IF
种属反应 : Rabbit
Wang AM et al. Oncotarget 2014; 5 (13):5002-16 Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.
实验应用 : WB
种属反应 : Human
Isayev O et al. Oncotarget 2014; 5 (13):5177-89 inhibition of glucose turnover by 3-bromopyruvate counteracts pancreatic cancer stem cell features and sensitizes cells to *.
实验应用 : IHC-Fr
种属反应 : Human
Liao CJ et al. BMC Cancer 2014; 14:555 Glucose-regulated protein 58 modulates β-catenin protein stability in a cervical adenocarcinoma cell line.
实验应用 : WB
种属反应 : Human
Jin L et al. Animal Cells and Systems 2014; (Epub) Expansion, characterization, and differentiation of rabbit bone marrow-derived mesenchymal stem cells in serum-free medium
实验应用 : ICC/IF
种属反应 : Rabbit
Ma L et al. Biochem Biophys Res Commun 2014; (Epub) Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits.
实验应用 : WB
种属反应 : Human
Xie G et al. Br J Cancer 2014; Tumour-initiating capacity is independent of epithelial-mesenchymal transition status in breast cancer cell lines.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
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