产品说明
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Tonsil: All lymphocytes and histiocytes must show a strong membranous CD45 staining.
Negative Control
Tonsil: Squamous epithelial cells must be completely CD45 negative.
产品属性
形式
Liquid
存储溶液
PBS, 0.05% BSA (Please contact us for PBS only format)
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human CD45 protein
纯化方式
Protein A/G purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200
细胞定位
Cell membrane
功能与背景
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
数据库
研究领域
產品图片
|
GTX04432 IHC-P Image
IHC-P analysis of human lymph node tissue section using GTX04432 CD45 antibody [MSVA-045R] HistoMAX™. In the lymph node virtually all hematolymphoid cells are CD45 positive.
|
|
GTX04432 IHC-P Image
IHC-P analysis of human appendix tissue section using GTX04432 CD45 antibody [MSVA-045R] HistoMAX™. In the appendix, CD45 immunostaining is stronger in lymphocytes than in macrophages. Epithelial cells are completely CD45 negative.
|
|
GTX04432 IHC-P Image
IHC-P analysis of human diffuse large B-cell lymphoma tissue section using GTX04432 CD45 antibody [MSVA-045R] HistoMAX™. Diffuse large B cell lymphoma showing strong CD45 staining of tumor cells.
|
文献引用
目前没有使用 CD45 antibody [MSVA-045R] HistoMAX™ (GTX04432) 的文献被收录。快来回报我们第一篇参考文献吧!
产品评论
目前没有使用 CD45 antibody [MSVA-045R] HistoMAX™ (GTX04432) 的产品评论被收录。快发表第一篇产品评论吧!