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CD63抗体 [MEM-259]

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219
Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219
Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219
Anti-CD63 antibody [MEM-259] used in Flow cytometry (FACS). GTX28219

目录号 GTX28219

文献引用 12

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 MEM-259

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

种属反应

 Human
产品包装
100 μg (¥4700)
查看所有 CD63 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
ICC/IF 10 μg/ml
IHC-P 10 μg/ml
FACS 2 μg/ml
IP Assay dependent
ELISA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

26 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

HPB-ALL T cell line

纯化方式

Protein A purified

偶联

Unconjugated

RRID

AB_374496

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CD63 , CD63 molecule , LAMP3 , ME491 , MLA1 , OMA81H , TSPAN30

细胞定位

Cell membrane,Lysosome membrane,Late endosome membrane,Endosome,Multivesicular body,Melanosome

功能与背景

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

数据库

研究领域

產品图片

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219

GTX28219 ICC/IF Image

ICC/IF analysis of human primary fibroblasts using GTX28219 CD63 antibody [MEM-259].
Green : Primary antibody
Red : Actin
Blue : DAPI

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219

GTX28219 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX28219 CD63 antibody [MEM-259].
Green : Primary antibody
Red : Actin
Blue : DAPI

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219

GTX28219 ICC/IF Image

ICC/IF analysis of human skin fibroblasts using GTX28219 CD63 antibody [MEM-259].
Green : Primary antibody
Red : Transferrin
Blue : DAPI

Anti-CD63 antibody [MEM-259] used in Flow cytometry (FACS). GTX28219

GTX28219 FACS Image

FACS analysis of IgE-activated peripheral blood using GTX28219 CD63 antibody [MEM-259].

文献引用

Application Reference

Afrodit? N?meth et al. Research Square 2023; Extracellular vesicles promote migration despite vemurafenib treatment in malignant melanoma cells
实验应用 : ICC/IF
Lin SJ et al. Nat Commun 2023; 14 (1) : 7859 Endosomal Arl4A attenuates EGFR degradation by binding to the ESCRT-II component VPS36.
Lanna A et al. Nat Cell Biol 2022; 24 (10) : 1461-1474 An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.
实验应用 : ELISA
种属反应 : Human
Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4) : e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.

Clone Reference

Huang C.C. et al. Microfluidics and Nanofluidics 2021; 25 A miniaturized, DNA-FET biosensor-based microfluidic system for quantification of two breast cancer biomarkers.
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Application Reference
Afrodit? N?meth et al. Research Square 2023; Extracellular vesicles promote migration despite vemurafenib treatment in malignant melanoma cells
实验应用 : ICC/IF
Lin SJ et al. Nat Commun 2023; 14 (1):7859 Endosomal Arl4A attenuates EGFR degradation by binding to the ESCRT-II component VPS36.
Lanna A et al. Nat Cell Biol 2022; 24 (10):1461-1474 An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.
实验应用 : ELISA
种属反应 : Human
Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4):e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.
Peng X et al. J Exp Clin Cancer Res 2021; 40 (1):183 LINC00511 drives invasive behavior in hepatocellular carcinoma by regulating exosome secretion and invadopodia formation.
实验应用 : ICC/IF
种属反应 : Human
Peng X et al. Biochim Biophys Acta Mol Cell Res 2021; 1868 (1):118857 IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.
实验应用 : WB, ICC/IF
种属反应 : Human
Yang L et al. Mol Cancer 2019; 18 (1):78 Long non-coding RNA HOTAIR promotes exosome secretion by regulating RAB35 and SNAP23 in hepatocellular carcinoma.
Raulf N et al. Eur J Cancer 2018; 102:52-68 Annexin A1 regulates EGFR activity and alters EGFR-containing tumour-derived exosomes in head and neck cancers.
实验应用 : WB
种属反应 : Human
Clone Reference
Lenzini S et al. ACS Nano 2021; 15 (11):17439-17452 Cell-Matrix Interactions Regulate Functional Extracellular Vesicle Secretion from Mesenchymal Stromal Cells.
Huang C.C. et al. Microfluidics and Nanofluidics 2021; 25 A miniaturized, DNA-FET biosensor-based microfluidic system for quantification of two breast cancer biomarkers.
Jakhria T et al. J Biol Chem 2014; β2-microglobulin Amyloid Fibrils are Nanoparticles that Disrupt Lysosomal Membrane Protein Trafficking and Inhibit Protein Degradation by Lysosomes.
Schmidt H et al. BMC Immunol 2009; 10:41 Enrichment and analysis of secretory lysosomes from lymphocyte populations.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 4700
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