产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human CD66e.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
carcinoembryonic antigen related cell adhesion molecule 5 , CD66e , CEA
细胞定位
Cell membrane,Apical cell membrane,Cell surface
功能与背景
This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
数据库
研究领域
產品图片
![Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816](/templates/default/js/lightbox/images/loading.gif "Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816")
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GTX638816 WB Image
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD66e antibody [HL2467] (GTX638816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638816 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD66e antibody [HL2467] (GTX638816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638816 IHC-P Image
CD66e antibody [HL2467] detects CD66e protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.CD66e stained by CD66e antibody [HL2467] (GTX638816) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816](/upload/website/prouct_img/small/GTX638816/GTX638816_45159_20230908_WB_treatment_PNGaseF_23091319_647.webp "Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816")
![Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816](/upload/website/prouct_img/small/GTX638816/GTX638816_T-45096_20230714_WB_TPM_watermark_23071822_766.webp "Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816")
![Anti-CD66e antibody [HL2467] used in IHC (Paraffin sections) (IHC-P). GTX638816](/upload/website/prouct_img/small/GTX638816/GTX638816_T-45096_20230721_IHC-P_23073119_681.webp "Anti-CD66e antibody [HL2467] used in IHC (Paraffin sections) (IHC-P). GTX638816")
![Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816](/upload/website/prouct_img/normal/GTX638816/GTX638816_45159_20230908_WB_treatment_PNGaseF_23091319_647.webp "Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816")
![Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816](/upload/website/prouct_img/normal/GTX638816/GTX638816_T-45096_20230714_WB_TPM_watermark_23071822_766.webp "Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816")
![Anti-CD66e antibody [HL2467] used in IHC (Paraffin sections) (IHC-P). GTX638816](/upload/website/prouct_img/normal/GTX638816/GTX638816_T-45096_20230721_IHC-P_23073119_681.webp "Anti-CD66e antibody [HL2467] used in IHC (Paraffin sections) (IHC-P). GTX638816")
