产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human CD73.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
5''-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT
功能与背景
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
数据库
研究领域
產品图片
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/templates/default/js/lightbox/images/loading.gif "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
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GTX638851 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638851 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX638851 IHC-P Image
CD73 antibody [HL2493] detects CD73 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.CD73 stained by CD73 antibody [HL2493] (GTX638851) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638851 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638851 WB Image
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/small/GTX638851/GTX638851_T-45110_20230721_WB_shRNA_watermark_23072519_694.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/small/GTX638851/GTX638851_T-45110_20230721_WB_M_R_23072519_495.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
![Anti-CD73 antibody [HL2493] used in IHC (Paraffin sections) (IHC-P). GTX638851](/upload/website/prouct_img/small/GTX638851/GTX638851_45173_20231020_IHC-P_23102401_165.webp "Anti-CD73 antibody [HL2493] used in IHC (Paraffin sections) (IHC-P). GTX638851")
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/small/GTX638851/GTX638851_45173_20231006_WB_TPM_watermark_23102401_597.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/small/GTX638851/GTX638851_45173_20230922_WB_Z_23100118_253.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/normal/GTX638851/GTX638851_T-45110_20230721_WB_shRNA_watermark_23072519_694.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/normal/GTX638851/GTX638851_T-45110_20230721_WB_M_R_23072519_495.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
![Anti-CD73 antibody [HL2493] used in IHC (Paraffin sections) (IHC-P). GTX638851](/upload/website/prouct_img/normal/GTX638851/GTX638851_45173_20231020_IHC-P_23102401_165.webp "Anti-CD73 antibody [HL2493] used in IHC (Paraffin sections) (IHC-P). GTX638851")
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/normal/GTX638851/GTX638851_45173_20231006_WB_TPM_watermark_23102401_597.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
![Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851](/upload/website/prouct_img/normal/GTX638851/GTX638851_45173_20230922_WB_Z_23100118_253.webp "Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851")
