产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human CD81 Extracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human CD81. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28
细胞定位
Cell membrane
功能与背景
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
数据库
研究领域
產品图片
![Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265](/templates/default/js/lightbox/images/loading.gif "Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265")
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GTX637265 WB Image
SU86.86 exsosome extracts (1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1667] (GTX637265) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637265 WB Image
Unboiled Jurkat membrane extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1667] (GTX637265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637265 ICC/IF Image
CD81 antibody [HL1667] detects CD81 protein by immunofluorescent analysis.Sample: Jurkat and HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD81 stained by CD81 antibody [HL1667] (GTX637265) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265](/upload/website/prouct_img/small/GTX637265/GTX637265_44879_20221202_WB_Fraction_22120719_417.webp "Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265")
![Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265](/upload/website/prouct_img/small/GTX637265/GTX637265_44879_20221209_WB_Fraction_22121123_408.webp "Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265")
![Anti-CD81 antibody [HL1667] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637265](/upload/website/prouct_img/small/GTX637265/GTX637265_T-44753_20221007_ICC_IF_22102723_131.webp "Anti-CD81 antibody [HL1667] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637265")
![Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265](/upload/website/prouct_img/normal/GTX637265/GTX637265_44879_20221202_WB_Fraction_22120719_417.webp "Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265")
![Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265](/upload/website/prouct_img/normal/GTX637265/GTX637265_44879_20221209_WB_Fraction_22121123_408.webp "Anti-CD81 antibody [HL1667] used in Western Blot (WB). GTX637265")
![Anti-CD81 antibody [HL1667] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637265](/upload/website/prouct_img/normal/GTX637265/GTX637265_T-44753_20221007_ICC_IF_22102723_131.webp "Anti-CD81 antibody [HL1667] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637265")
