CD81抗体

Name: CD81抗体
Brand: GeneTex
SKU: GTX101766
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-CD81 antibody used in Western Blot (WB). GTX101766

Anti-CD81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101766

Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX101766

Anti-CD81 antibody used in Flow cytometry (FACS). GTX101766

目录号 GTX101766

文献引用 20

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, FACS, Blocking
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 CD81 的产品

View product citations for antibody GTX101766 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
FACS	1:50-1:200
Blocking	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

26 kDa. ( Note )

预测种属反应

Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618892

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

细胞定位

Membrane

功能与背景

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]

数据库

Gene ID: 975 CD81
UniProt: P60033 CD81

研究领域

產品图片

GTX101766 WB Image

AsPC-1 exosome extracts (5.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101766 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101766 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101766 ICC/IF Image

CD81 antibody detects CD81 protein by immunofluorescent analysis.
Sample: Jurkat and HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD81 stained by CD81 antibody (GTX101766) diluted at 1:500.

GTX101766 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using CD81(GTX101766) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101766 FACS Image

CD81 antibody (GTX101766) detects CD81 by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD81 antibody (GTX101766) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX101766 WB Image

The data was published in the journal J Clin Med in 2019.PMID: 31167519

GTX101766 WB Image

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

GTX101766 WB Image

The data was published in the journal J Hematol Oncol in 2019. PMID: 30683126

文献引用

Application Reference

Duan TQ et al. Neural Regen Res 2023; 18 (10) : 2315-2320 Adipose mesenchymal stem cell-derived extracellular vesicles reduce glutamate-induced excitotoxicity in the retina.

Tong L et al. Sci Adv 2023; 9 (15) : eade5041 Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis.

实验应用 : WB

种属反应 : Human

Liu Q et al. Nutrients 2023; 15 (3) Oral Administration of Bovine Milk-Derived Extracellular Vesicles Attenuates Cartilage Degeneration via Modulating Gut Microbiota in DMM-Induced Mice.

实验应用 : WB

种属反应 : Mouse

Ramos EK et al. Elife 2022; 11 Machine learning-assisted elucidation of CD81-CD44 interactions in promoting cancer stemness and extracellular vesicle integrity.

实验应用 : WB

种属反应 : Human

Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.

实验应用 : WB

种属反应 : Human

显示更多 +

我要回报文献

产品评论

CD81 antibody 目录号 GTX101766

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 2 users reviews)

Date : Anonymous submitted on 25-Dec-2020

Score :

Application Tested : WB

Sample Species : Ms

Sample : B16F10 cells

Amount used : 10 μg

Blocking : 0.5% skim milk in TBST, 25°C, 1Hr

Primary Antibody : 1:5000, 25°C, 12Hr

Date : Anonymous submitted on 25-May-2018

Score :

Application Tested : WB

Sample Species : Hu

Sample : AGS cells

Amount used : 15 μg

Blocking : 5% milk, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

我要评论产品

Application Reference

Duan TQ et al. Neural Regen Res 2023; 18 (10):2315-2320 Adipose mesenchymal stem cell-derived extracellular vesicles reduce glutamate-induced excitotoxicity in the retina.

Tong L et al. Sci Adv 2023; 9 (15):eade5041 Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis.

实验应用 : WB

种属反应 : Human

Liu Q et al. Nutrients 2023; 15 (3) Oral Administration of Bovine Milk-Derived Extracellular Vesicles Attenuates Cartilage Degeneration via Modulating Gut Microbiota in DMM-Induced Mice.

实验应用 : WB

种属反应 : Mouse

Ramos EK et al. Elife 2022; 11 Machine learning-assisted elucidation of CD81-CD44 interactions in promoting cancer stemness and extracellular vesicle integrity.

实验应用 : WB

种属反应 : Human

Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.

实验应用 : WB

种属反应 : Human

Tong L et al. Theranostics 2021; 11 (17):8570-8586 Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

实验应用 : WB

种属反应 : Mouse

Xinhui Liu et al. Front Pharmacol 2020; 11:622658 Involvement of Circulating Exosomal MicroRNAs in Jian-Pi-Yi-Shen Formula Protection Against Adenine-Induced Chronic Kidney Disease.

实验应用 : WB

种属反应 : Rat

Imai A et al. Odontology 2020; Comprehensive analysis and comparison of proteins in salivary exosomes of climacteric and adolescent females.

实验应用 : WB

种属反应 : Human

Tong L et al. Mol Nutr Food Res 2020; 64 (8):e1901251 Oral Administration of Bovine Milk-Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice.

实验应用 : WB

种属反应 : Mouse

Xue Y et al. Am J Pathol 2020; 190 (7):1427-1437 Phosphorylated Ezrin (Thr567) Regulates Hippo Pathway and Yes-Associated Protein (Yap) in Liver.

实验应用 : WB

种属反应 : Human

Chen SY et al. Stem Cells Transl Med 2020; 9 (4):499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.

实验应用 : WB

种属反应 : Mouse

Huang WT et al. Cancer Lett 2019; 442:287-298 Pigment epithelium-derived factor inhibits lung cancer migration and invasion by upregulating exosomal thrombospondin 1.

实验应用 : WB, ICC/IF

种属反应 : Human

Kuo HF et al. J Clin Med 2019; 8 (6) Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion.

Cheng WC et al. Int J Cancer 2019; (Epub) RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.

实验应用 : WB

种属反应 : Human

Hwang WL et al. J Hematol Oncol 2019; 12 (1):10 Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer.

实验应用 : WB

Pascua-Maestro R et al. Front Cell Neurosci 2018; 12:526 Extracellular Vesicles Secreted by Astroglial Cells Transport Apolipoprotein D to Neurons and Mediate Neuronal Survival Upon Oxidative Stress.

实验应用 : WB

种属反应 : Human

Xue Y et al. Am J Pathol 2018; (Epub) Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes.

实验应用 : WB

种属反应 : Human

Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.

实验应用 : WB

种属反应 : Human

Lin MC et al. J Vis Exp 2017; (124) Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes.

实验应用 : WB

Deng A et al. Virus Res 2014; 184:93-102 Human immunodeficiency virus type 1 Vpr increases hepatitis C virus RNA replication in cell culture.

实验应用 : Blocking

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

	(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
	http://www.neobioscience.com
	genetex@neobioscience.com

CD81抗体

目录号 GTX101766

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX101766 WB Image

GTX101766 WB Image

GTX101766 WB Image

GTX101766 ICC/IF Image

GTX101766 IHC-P Image

GTX101766 FACS Image

GTX101766 WB Image

GTX101766 WB Image

GTX101766 WB Image

文献引用

Application Reference

产品评论

CD81 antibody 目录号 GTX101766

产品

研究

支持

公司

Follow Us

粤ICP备08007346号-9

© 1998-2022 GeneTex, Inc. 保留所有权利。

所有产品仅供研究使用 - 不应用于诊断或治疗。