产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human CD97 Extracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human CD97. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
adhesion G protein-coupled receptor E5 , CD97 , TM7LN1
细胞定位
Cell membrane; Multi-pass membrane protein
功能与背景
This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
数据库
研究领域
產品图片
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/templates/default/js/lightbox/images/loading.gif "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
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GTX637673 WB Image
Boiled and unboiled wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637673 ICC/IF Image
CD97 antibody [HL1924] detects CD97 protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis.Sample: U937 cells were fixed in ice-cold MeOH for 5 min.Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637673 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637673 ICC/IF Image
CD97 antibody [HL1924] detects CD97 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in MeOH.Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637673 WB Image
Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637673 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637673 IHC-P Image
CD97 antibody [HL1924] detects CD97 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human lung.CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/small/GTX637673/GTX637673_T-44837_20231006_WB_KO_watermark_23102401_836.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673](/upload/website/prouct_img/small/GTX637673/GTX637673_T-44837_20230714_ICC_IF_23072519_958.webp "Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673")
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/small/GTX637673/GTX637673_45166_20240112_WB_TPM_watermark_24011618_239.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673](/upload/website/prouct_img/small/GTX637673/GTX637673_45166_20231229_ICC_IF_B_24011618_433.webp "Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673")
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/small/GTX637673/GTX637673_45166_20230915_WB_B_23091901_981.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/small/GTX637673/GTX637673_45166_20230922_WB_D_23100118_388.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in IHC (Paraffin sections) (IHC-P). GTX637673](/upload/website/prouct_img/small/GTX637673/GTX637673_T-44837_20230928_IHC-P_23100319_734.webp "Anti-CD97 antibody [HL1924] used in IHC (Paraffin sections) (IHC-P). GTX637673")
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/normal/GTX637673/GTX637673_T-44837_20231006_WB_KO_watermark_23102401_836.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673](/upload/website/prouct_img/normal/GTX637673/GTX637673_T-44837_20230714_ICC_IF_23072519_958.webp "Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673")
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20240112_WB_TPM_watermark_24011618_239.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673](/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20231229_ICC_IF_B_24011618_433.webp "Anti-CD97 antibody [HL1924] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637673")
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20230915_WB_B_23091901_981.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673](/upload/website/prouct_img/normal/GTX637673/GTX637673_45166_20230922_WB_D_23100118_388.webp "Anti-CD97 antibody [HL1924] used in Western Blot (WB). GTX637673")
![Anti-CD97 antibody [HL1924] used in IHC (Paraffin sections) (IHC-P). GTX637673](/upload/website/prouct_img/normal/GTX637673/GTX637673_T-44837_20230928_IHC-P_23100319_734.webp "Anti-CD97 antibody [HL1924] used in IHC (Paraffin sections) (IHC-P). GTX637673")
