Wild-type (WT) and CD97 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and competitor's antibody (Clone EPR4427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Untreated (–) and treated (+) U937 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunoprecipitation of CD97 protein from U937 using 5 μg of CD97 antibody [HL1925] (GTX637674).
Western blot analysis was performed using CD97 antibody [HL1925] (GTX637674).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.