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CDK6抗体 [HL2078]

Recombinant antibody Orthogonal Overexpression
Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986
Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986
Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986
Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986
Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986

目录号 GTX637986

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2078

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

37 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human CDK6.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cyclin dependent kinase 6 , MCPH12 , PLSTIRE

细胞定位

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

功能与背景

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]

数据库

研究领域

產品图片

Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986

GTX637986 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [HL2078] (GTX637986) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986

GTX637986 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [HL2078] (GTX637986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986

GTX637986 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [HL2078] (GTX637986) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986

GTX637986 IHC-P Image

CDK6 antibody [HL2078] detects CDK6 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
CDK6 stained by CDK6 antibody [HL2078] (GTX637986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986

GTX637986 IHC-P Image

CDK6 antibody [HL2078] detects CDK6 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
CDK6 stained by CDK6 antibody [HL2078] (GTX637986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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