产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of Human CHD4.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
chromodomain helicase DNA binding protein 4 , CHD-4 , Mi-2b , Mi2-BETA , SIHIWES
细胞定位
Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
功能与背景
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
数据库
研究领域
產品图片
![Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450](/templates/default/js/lightbox/images/loading.gif "Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450")
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GTX637450 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody [HL1798] (GTX637450) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637450 IHC-P Image
CHD4 antibody [HL1798] detects CHD4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ductus deferens.
CHD4 stained by CHD4 antibody [HL1798] (GTX637450) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637450 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody [HL1798] (GTX637450) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450](/upload/website/prouct_img/small/GTX637450/GTX637450_45299_20240202_WB_24021917_444.webp "Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450")
![Anti-CHD4 antibody [HL1798] used in IHC (Paraffin sections) (IHC-P). GTX637450](/upload/website/prouct_img/small/GTX637450/GTX637450_T-44809_20231208_IHC-P_M_23121922_572.webp "Anti-CHD4 antibody [HL1798] used in IHC (Paraffin sections) (IHC-P). GTX637450")
![Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450](/upload/website/prouct_img/small/GTX637450/GTX637450_T-44809_20231117_WB_Fraction_23112021_464.webp "Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450")
![Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450](/upload/website/prouct_img/normal/GTX637450/GTX637450_45299_20240202_WB_24021917_444.webp "Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450")
![Anti-CHD4 antibody [HL1798] used in IHC (Paraffin sections) (IHC-P). GTX637450](/upload/website/prouct_img/normal/GTX637450/GTX637450_T-44809_20231208_IHC-P_M_23121922_572.webp "Anti-CHD4 antibody [HL1798] used in IHC (Paraffin sections) (IHC-P). GTX637450")
![Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450](/upload/website/prouct_img/normal/GTX637450/GTX637450_T-44809_20231117_WB_Fraction_23112021_464.webp "Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450")
