产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Sheep, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human CLOCK. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909961
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
clock circadian regulator , KAT13D , bHLHe8
细胞定位
Nucleus,Cytoplasm,Cytoplasm, cytosol
功能与背景
The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
数据库
研究领域
產品图片
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/templates/default/js/lightbox/images/loading.gif "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
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GTX636283 WB Image
Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636283 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:2000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636283 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:2000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636283 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (GTX636283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636283 IHC-P Image
CLOCK antibody [HL1099] detects CLOCK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.CLOCK stained by CLOCK antibody [HL1099] (GTX636283) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636283 ICC/IF Image
CLOCK antibody [HL1099] detects CLOCK protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CLOCK stained by CLOCK antibody [HL1099] (GTX636283) diluted at 1:500.Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/small/GTX636283/GTX636283_44375_20220128_WB_KO_watermark_w_23061202_442.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/small/GTX636283/GTX636283_44375_20220902_WB_M_competitor_watermark_22090701_266.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/small/GTX636283/GTX636283_44375_20220902_WB_competitor_watermark_22090701_521.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/small/GTX636283/GTX636283_44375_20210806_WB_treatment_Serum_w_23061202_245.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in IHC (Paraffin sections) (IHC-P). GTX636283](/upload/website/prouct_img/small/GTX636283/GTX636283_44375_20221227_IHC-P_multiple_M_22122821_404.webp "Anti-CLOCK antibody [HL1099] used in IHC (Paraffin sections) (IHC-P). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636283](/upload/website/prouct_img/small/GTX636283/GTX636283_44375_20211008_ICC_IF_R_w_23061202_691.webp "Anti-CLOCK antibody [HL1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/normal/GTX636283/GTX636283_44375_20220128_WB_KO_watermark_w_23061202_442.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/normal/GTX636283/GTX636283_44375_20220902_WB_M_competitor_watermark_22090701_266.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/normal/GTX636283/GTX636283_44375_20220902_WB_competitor_watermark_22090701_521.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283](/upload/website/prouct_img/normal/GTX636283/GTX636283_44375_20210806_WB_treatment_Serum_w_23061202_245.webp "Anti-CLOCK antibody [HL1099] used in Western Blot (WB). GTX636283")
![Anti-CLOCK antibody [HL1099] used in IHC (Paraffin sections) (IHC-P). GTX636283](/upload/website/prouct_img/normal/GTX636283/GTX636283_44375_20221227_IHC-P_multiple_M_22122821_404.webp "Anti-CLOCK antibody [HL1099] used in IHC (Paraffin sections) (IHC-P). GTX636283")
![Anti-CLOCK antibody [HL1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636283](/upload/website/prouct_img/normal/GTX636283/GTX636283_44375_20211008_ICC_IF_R_w_23061202_691.webp "Anti-CLOCK antibody [HL1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636283")
