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CNPase抗体

KO/KD Orthogonal
Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103954
Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954

目录号 GTX103954

文献引用 1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

阳性对照

mouse brain , rat brain , HeLa

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human CNPase. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036608

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2',3'-cyclic nucleotide 3' phosphodiesterase , CNP1

细胞定位

Membrane , Melanosome

数据库

研究领域

產品图片

Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

KO/KD

Wild-type (WT) and CNPase knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNPase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103954

GTX103954 ICC/IF Image

CNPase antibody detects CNPase protein at cell membrane by immunofluorescent analysis.
Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CNPase stained by CNPase antibody (GTX103954) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954

GTX103954 IHC-P Image

CNP antibody detects CNP protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
CNP antibody (GTX103954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954

GTX103954 IHC-P Image

CNPase antibody detects CNPase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CNPase stained by CNPase antibody (GTX103954) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNP antibody (GTX103954) diluted at 1:5000.

Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954

GTX103954 IHC-P Image

CNPase antibody detects CNPase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CNPase stained by CNPase antibody (GTX103954) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

KO/KD Orthogonal

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

文献引用

Application Reference

Chen SY et al. Stem Cells Transl Med 2020; 9 (4) : 499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
实验应用 : WB
种属反应 : Mouse
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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