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COL1A1抗体 [N1N2], N-term

Orthogonal
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

目录号 GTX112731

文献引用 21
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IP

种属反应

 Polyodon spathula, Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 COL1A1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

139 kDa. ( Note )

预测种属反应

Bovine, Dog, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human COL1A1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10721155

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4

细胞定位

Secreted , extracellular space , extracellular matrix

功能与背景

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

数据库

研究领域

產品图片

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL1A1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112731

GTX112731 IP Image

Immunoprecipitation of COL1A1 protein from SK-N-AS whole cell extracts using 5 μg of COL1A1 antibody [N1N2], N-term (GTX112731).
Western blot analysis was performed using COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL1A1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112731

GTX112731 ICC/IF Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at vesicle by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-COL1A1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112731

GTX112731 ICC/IF Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at vesicle by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

Orthogonal

The data was published in the journal J Cell Mol Med in 2020.PMID: 31957232

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

GTX112731 IHC-P Image

Orthogonal

The data was published in the journal J Cell Mol Med in 2020.PMID: 31957232

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

GTX112731 WB Image

Orthogonal

The data was published in the journal J Cell Mol Med in 2020.PMID: 31957232

文献引用

Application Reference

Liu SH et al. Open Life Sci 2024; 19 (1) : 20220828 Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging.
Idelfonso-Garc?a OG et al. Antioxidants (Basel) 2024; 13 (3) Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver.
OG Idelfonso-Garc?a et al. Antioxidants? 2024; 13 (1) : 257 Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver
实验应用 : IHC-P
Jiang L et al. Front Bioeng Biotechnol 2024; 12 : 1302223 Microfluidic-based human prostate-cancer-on-chip.
Chiou WC et al. Biomed Pharmacother 2023; 161 : 114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
实验应用 : WB
种属反应 : Human
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COL1A1 antibody [N1N2], N-term 目录号 GTX112731

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Date : Anonymous submitted on 06-Feb-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human dental pulp stem cells
Amount used : 5μg
Blocking : 3%BSA in TBS-T, 24°C, 1Hr
Primary Antibody : 1:1000, 4°C, 14Hr
Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731
Date : Anonymous submitted on 02-Jul-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : NIH3T3 cells
Amount used : 15μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
Application Reference
Liu SH et al. Open Life Sci 2024; 19 (1):20220828 Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging.
Idelfonso-Garc?a OG et al. Antioxidants (Basel) 2024; 13 (3) Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver.
OG Idelfonso-Garc?a et al. Antioxidants? 2024; 13 (1):257 Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver
实验应用 : IHC-P
Jiang L et al. Front Bioeng Biotechnol 2024; 12:1302223 Microfluidic-based human prostate-cancer-on-chip.
Chiou WC et al. Biomed Pharmacother 2023; 161:114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
实验应用 : WB
种属反应 : Human
Li LX et al. Cells 2022; 12 (1) Single-Cell and CellChat Resolution Identifies Collecting Duct Cell Subsets and Their Communications with Adjacent Cells in PKD Kidneys.
Russell DF et al. PLoS One 2022; 17 (11):e0276854 Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon.
实验应用 : IHC-Fr
种属反应 : Polyodon spathula
Ching-Chung Ko et al. Int J Mol Sci 2022; 23 (12) Long Non-Coding RNA MIR31HG Promotes the Transforming Growth Factor β-Induced Epithelial-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma Cells.
实验应用 : WB
种属反应 : Human
Yongchang Yang et al. Front Genet 2021; 12:831420 Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation.
实验应用 : WB
种属反应 : Mouse
Seongki Min et al. Cell Death Dis 2021; 12 (11):1076 MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness.
实验应用 : WB
种属反应 : Human
Chiang YF et al. Nutrients 2021; 13 (9) The Role of Cell Proliferation and Extracellular Matrix Accumulation Induced by Food Additive Butylated Hydroxytoluene in Uterine Leiomyoma.
实验应用 : WB
种属反应 : Rat
Besson B et al. PLoS One 2020; 15 (5):e0232585 Comparison of a human neuronal model proteome upon Japanese encephalitis or West Nile Virus infection and potential role of mosquito saliva in neuropathogenesis.
实验应用 : WB
种属反应 : Human
Mohankumar K et al. Endocrinology 2020; 161 (4) Bis-Indole-Derived Nuclear Receptor 4A1 (NR4A1, Nur77) Ligands as Inhibitors of Endometriosis.
实验应用 : WB
种属反应 : Human
Liu T et al. J Cell Mol Med 2020; 24 (3):2189-2201 Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA-328-3p-mediated inhibition of COL1A1.
实验应用 : WB
种属反应 : Human
Wu PT et al. J Biol Chem 2019; 294 (52):20177-20184 Inhibition of CD44 induces apoptosis, inflammation, and matrix metalloproteinase expression in tendinopathy.
Choong OK et al. Theranostics 2019; 9 (22):6550-6567 Hypoxia-induced H19/YB-1 cascade modulates cardiac remodeling after infarction.
实验应用 : WB, ICC/IF
种属反应 : Mouse
Chen HY et al. Antioxidants (Basel) 2019; 8 (4) Natural Antioxidant Resveratrol Suppresses Uterine Fibroid Cell Growth and Extracellular Matrix Formation In Vitro and In Vivo.
实验应用 : WB
种属反应 : Human
Chen HY et al. Cell Physiol Biochem 2018; 49 (5):1970-1986 Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression.
实验应用 : WB
种属反应 : Rat
Stroo I et al. BMC Nephrol 2018; 19 (1):78 No difference in renal injury and fibrosis between wild-type and NOD1/NOD2 double knockout mice with chronic kidney disease induced by ureteral obstruction.
实验应用 : IHC-P
种属反应 : Mouse
Chen S et al. World J Urol 2016; (Epub) Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway.
实验应用 : WB
种属反应 : Human
Araki M et al. Sci Rep 2016; 6:31216 Conditional Deletion of Smad1 Ameliorates Glomerular Injury in Progressive Glomerulonephritis.
实验应用 : IHC-P
种属反应 : Mouse
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Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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