COL1A2抗体 [C2C3], C-term

Name: COL1A2抗体 [C2C3], C-term
Brand: GeneTex
SKU: GTX102996
Price: 4000.00 RMB

Anti-COL1A2 antibody [C2C3], C-term used in Western Blot (WB). GTX102996

Anti-COL1A2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102996

目录号 GTX102996

文献引用 6

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF
种属反应	Human, Mouse, Zebrafish
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 COL1A2 的产品

View product citations for antibody GTX102996 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
ICC/IF	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

129 kDa. ( Note )

预测种属反应

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human COL1A2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950011

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

collagen type I alpha 2 chain , EDSARTH2 , EDSCV , OI4

细胞定位

Secreted , extracellular space , extracellular matrix

功能与背景

This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

数据库

Gene ID: 1278 COL1A2
UniProt: P08123 COL1A2

研究领域

產品图片

GTX102996 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Col1a2 antibody (GTX102996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102996 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102996 WB Image

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102996 ICC/IF Image

COL1A2 antibody [C2C3], C-term detects COL1A2 protein at cytoplasm by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in ice-cold MeOH for 5 min.
Green: COL1A2 protein stained by COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102996 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.